Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 3
PLOD2 Antibody (PA5-110181) in WB
Western blot analysis of PLOD2 in extracts of various cell lines. Samples were incubated with PLOD2 Polyclonal antibody (Product # PA5-110181) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
产品信息
PA5-110181
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 260-380 of human PLOD2/LH2 (NP_0009262)
偶联物
形式
Liquid
浓度
0.8 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2855592
产品详细信息
Immunogen sequence: ILLNYFGNYV PNSWTQDNGC TLCEFDTVDL SAVDVHPNVS IGVFIEQPTP FLPRFLDILL TLDYPKEALK LFIHNKEVYH EKDIKVFFDK AKHEIKTIKI VGPEENLSQA EARNMGMDFC R
靶标信息
The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: LH2; lysine hydroxylase 2; lysyl hydroxlase 2; Lysyl hydroxylase 2; Procollagen-lysine 2-oxoglutarate 5-dioxygenase (Lysine hydroxylase) 2; procollagen-lysine 5-dioxygenase; Procollagen-lysine, 2-oxoglutarate 5-dioxygenase (Lysine hydroxylase) 2; procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2; Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2; telopeptide lysyl hydroxylase
基因别名: BRKS2; D530025C14Rik; LH2; Plod-2; PLOD2; TLH
UniProt ID: (Human) O00469, (Mouse) Q9R0B9
Entrez Gene ID: (Human) 5352, (Rat) 300901, (Mouse) 26432
iron ion binding
procollagen-lysine 5-dioxygenase activity
L-ascorbic acid binding
molecular_function
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
metal ion binding
dioxygenase activity
protein modifying enzyme
