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PLOD3 Antibody (PA5-87976) in IHC (P)
Immunohistochemistry analysis of PLOD3 in paraffin-embedded mouse heart. Samples were incubated with PLOD3 Polyclonal antibody (Product # PA5-87976) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
产品信息
PA5-87976
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human PLOD3 (NP_0010751)
偶联物
形式
Liquid
浓度
0.96 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2804557
产品详细信息
Immunogen sequence: SDRPRGRDPV NPEKLLVITV ATAETEGYLR FLRSAEFFNY TVRTLGLGEE WRGGDVARTV GGGQKVRWLK KEMEKYADRE DMIIMFVDSY DVILAGSPTE LLKKFVQSGS RLLFSAESFC WPEWGLAEQY PEVGTGKRFL NSGGFIGFAT TIHQIVRQWK YKDDDDDQLF YTRLYLDPGL REKLSLNLDH KSRIFQNLNG ALDEVVLKFD RNRVRIRNVA YDTLPIVVHG NGPTKLQLNY LGNYVPNGWT PEGGCG; Positive Samples: MCF-7, H460, 22RV1, HepG2, Mouse lung, Rat liver, Rat kidney; Cellular Location: Lumenal side, Peripheral membrane protein, Rough endoplasmic reticulum membrane
靶标信息
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: lysine hydroxylase 3; lysyl hydroxlase 3; lysyl hydroxylase 2; lysyl hydroxylase 3; Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3; procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3; procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
基因别名: AI414586; LH3; PLOD3
UniProt ID: (Human) O60568, (Mouse) Q9R0E1
Entrez Gene ID: (Human) 8985, (Mouse) 26433
iron ion binding
protein binding
procollagen-lysine 5-dioxygenase activity
L-ascorbic acid binding
procollagen glucosyltransferase activity
procollagen galactosyltransferase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
metal ion binding
dioxygenase activity
protein modifying enzyme
