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PLOD3 Antibody (MA5-51264) in WB
Western blot analysis of PLOD3 in extracts of various cell lines. Samples were incubated with PLOD3 Recombinant monoclonal antibody (Product # MA5-51264) using a dilution of 1:2,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
产品信息
MA5-51264
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
5R6Q9
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 25-306 of human PLOD3 (NP_001075.1).
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
内含物
0.05% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_3093866
产品详细信息
Immunogen sequence: SDRPRGRDPV NPEKLLVITV ATAETEGYLR FLRSAEFFNY TVRTLGLGEE WRGGDVARTV GGGQKVRWLK KEMEKYADRE DMIIMFVDSY DVILAGSPTE LLKKFVQSGS RLLFSAESFC WPEWGLAEQY PEVGTGKRFL NSGGFIGFAT TIHQIVRQWK YKDDDDDQLF YTRLYLDPGL REKLSLNLDH KSRIFQNLNG ALDEVVLKFD RNRVRIRNVA YDTLPIVVHG NGPTKLQLNY LGNYVPNGWT PEGGCGFCNQ DRRTLPGGQP PPRVFLAVFV EQ
靶标信息
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: lysine hydroxylase 3; lysyl hydroxlase 3; lysyl hydroxylase 2; lysyl hydroxylase 3; Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3; procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3; procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
基因别名: AI414586; LH3; PLOD3
UniProt ID: (Human) O60568, (Mouse) Q9R0E1
Entrez Gene ID: (Human) 8985, (Mouse) 26433
iron ion binding
protein binding
procollagen-lysine 5-dioxygenase activity
L-ascorbic acid binding
procollagen glucosyltransferase activity
procollagen galactosyltransferase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
metal ion binding
dioxygenase activity
protein modifying enzyme
