Invitrogen

PLZF Monoclonal Antibody (Mags.21F7), Alexa Fluor™ 488, eBioscience™

8 Published Figures

11 References

View all (19) PLZF antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

FIGURE: 1 / 9

PLZF Antibody (53-9320-82) in Flow

C57Bl/6 thymocytes were surface-stained with Anti-Mouse TCR beta, Anti-Mouse CD1d Tetramer, and Anti-Mouse NK1-1 PerCP-Cyanine5-5 (Product # 45-5941-82), followed by intracellular staining with Anti-Human/Mouse PLZF Alexa Fluor® 488 using the Foxp3 Staining Buffer Set and protocol (Product # 00-5523-00). Anti-Human/Mouse PLZF Alexa Fluor® 488 staining (right) was compared in T cells (CD1d Tetramer-NK1-1-TCR beta+; blue histogram), NKT1 cells (CD1d Tetramer+NK1-1+TCR beta+; orange histogram), and NKT2 cells (CD1d Tetramer+NK1-1-TCR beta+; p... View More

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Product Details

53-9320-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Mags.21F7

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2574445

Product Specific Information

Description: This Mags.21F7 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans. In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells. Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells. In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells. PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, and growth suppression. Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor.

Applications Reported: This Mags.21F7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This Mags.21F7 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

NKT cells adopt a glutamine-addicted phenotype to regulate their homeostasis and function.

Cell reports

Kumar A,Yarosz EL,Andren A,Zhang L,Lyssiotis CA,Chang CH

53-9320-82 was used in Flow cytometry/Cell sorting to highlight a distinct metabolic requirement of NKT cells compared with CD4 T cells, which may have therapeutic implications in the treatment of certain nutrient-restricted diseases.

Tue Oct 25 00:00:00 UTC 2022

Loss of Ubiquitin Carboxy-Terminal Hydrolase L1 Impairs Long-Term Differentiation Competence and Metabolic Regulation in Murine Spermatogonial Stem Cells.

Cells

Alpaugh WF,Voigt AL,Dardari R,Su L,Al Khatib I,Shin W,Goldsmith TM,Coyle KM,Tang LA,Shutt TE,Klein C,Biernaskie J,Dobrinski I

Published figure using PLZF monoclonal antibody (Product # 53-9320-82) in Flow Cytometry

Tue Aug 31 00:00:00 UTC 2021

Cytotoxicity of nonylphenol on spermatogonial stem cells via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin pathway.

World journal of stem cells

Lei JH,Yan W,Luo CH,Guo YM,Zhang YY,Wang XH,Su XJ

53-9320 was used in Flow cytometry/Cell sorting to investigate the cytotoxic effect of NP on SSCs via the phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) pathway.

Fri Jun 26 00:00:00 UTC 2020

Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.

Nature communications

Harsha Krovi S,Zhang J,Michaels-Foster MJ,Brunetti T,Loh L,Scott-Browne J,Gapin L

53-9320 was used in Flow cytometry/Cell sorting to highlight the common requirements for the development of innate-like T cells with a focus on how Hivep3 impacts the maturation of these lymphocytes.

Mon Dec 07 00:00:00 UTC 2020

Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.

Nature communications

Harsha Krovi S,Zhang J,Michaels-Foster MJ,Brunetti T,Loh L,Scott-Browne J,Gapin L

53-9320 was used in Flow cytometry/Cell sorting to highlight the common requirements for the development of innate-like T cells with a focus on how Hivep3 impacts the maturation of these lymphocytes.

Mon Dec 07 00:00:00 UTC 2020

Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells.

Nature communications

Lucas B,White AJ,Cosway EJ,Parnell SM,James KD,Jones ND,Ohigashi I,Takahama Y,Jenkinson WE,Anderson G

53-9320 was used in Flow cytometry/Cell sorting to study the mechanism that controls the development of αβ T cell lineages in the thymus.

Mon May 04 00:00:00 UTC 2020

Transnuclear mice reveal Peyer's patch iNKT cells that regulate B-cell class switching to IgG1.

The EMBO journal

Clancy-Thompson E,Chen GZ,LaMarche NM,Ali LR,Jeong HJ,Crowley SJ,Boelaars K,Brenner MB,Lynch L,Dougan SK

Published figure using PLZF monoclonal antibody (Product # 53-9320-82) in Flow Cytometry

Mon Jul 15 00:00:00 UTC 2019

In Vitro Cytotoxicity of Folate-Silica-Gold Nanorods on Mouse Acute Lymphoblastic Leukemia and Spermatogonial Cells.

Cell journal

Eslahi N,Shakeri-Zadeh A,Ashtari K,Pirhajati-Mahabadi V,Tohidi Moghadam T,Shabani R,Kamrava K,Madjd Z,Maki C,Asgari HR,Koruji M

53-9320 was used in Flow cytometry/Cell sorting to evaluate in vitro cytotoxicity of gold nanorods on the viability of spermatogonial cells and mouse acute lymphoblastic leukemia cells.

Mon Apr 01 00:00:00 UTC 2019

In Vitro Cytotoxicity of Folate-Silica-Gold Nanorods on Mouse Acute Lymphoblastic Leukemia and Spermatogonial Cells.

Cell journal

Eslahi N,Shakeri-Zadeh A,Ashtari K,Pirhajati-Mahabadi V,Tohidi Moghadam T,Shabani R,Kamrava K,Madjd Z,Maki C,Asgari HR,Koruji M

53-9320 was used in Flow cytometry/Cell sorting to evaluate in vitro cytotoxicity of gold nanorods on the viability of spermatogonial cells and mouse acute lymphoblastic leukemia cells.

Mon Apr 01 00:00:00 UTC 2019

Keap1-Nrf2 System Plays an Important Role in Invariant Natural Killer T Cell Development and Homeostasis.

Cell reports

Pyaram K,Kumar A,Kim YH,Noel S,Reddy SP,Rabb H,Chang CH

53-9320 was used in Flow cytometry/Cell sorting to report that T cellspecific deletion of Keap1 results in defective development and reduced numbers of invariant natural killer T (NKT) cells in the thymus and the peripheral organs in a cell-intrinsic manner.

Tue Apr 16 00:00:00 UTC 2019

Stabilization of cytokine mRNAs in iNKT cells requires the serine-threonine kinase IRE1alpha.

Nature communications

Govindarajan S,Gaublomme D,Van der Cruyssen R,Verheugen E,Van Gassen S,Saeys Y,Tavernier S,Iwawaki T,Bloch Y,Savvides SN,Lambrecht BN,Janssens S,Elewaut D,Drennan MB

53-9320 was used in Flow cytometry/Cell sorting to examine the role of the IRE1α-dependent signalling cascade in coupling constitutive cytokine mRNA expression to the rapid induction of cytokine secretion and effector functions in activated iNKT cells.

Mon Dec 17 00:00:00 UTC 2018

Monoclonal Invariant NKT (iNKT) Cell Mice Reveal a Role for Both Tissue of Origin and the TCR in Development of iNKT Functional Subsets.

Journal of immunology (Baltimore, Md. : 1950)

Clancy-Thompson E,Chen GZ,Tyler PM,Servos MM,Barisa M,Brennan PJ,Ploegh HL,Dougan SK

53-9320 was used in Flow cytometry/Cell sorting to examine how T cell receptor specificity determines invariant natural killer T cell function.

Sat Jul 01 00:00:00 UTC 2017

Distinct germline progenitor subsets defined through Tsc2-mTORC1 signaling.

EMBO reports

Hobbs RM,La HM,Mäkelä JA,Kobayashi T,Noda T,Pandolfi PP

53-9320 was used in Flow cytometry/Cell sorting to propose that mTORC1 is a key regulator of SPC fate and defines phenotypically distinct SPC subpopulations for self-renewal and differentiation.

Wed Apr 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Green's luxoid; luxoid; promyelocytic leukaemia zinc finger; Promyelocytic leukemia zinc finger protein; Zinc finger and BTB domain-containing protein 16; Zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein PLZF

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Gene Aliases: AI467657; lu; PLZF; ZBTB16; Zfp145; ZNF145

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UniProt ID: (Human) Q05516

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Entrez Gene ID: (Human) 7704, (Mouse) 235320

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Function(s)

RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding protein binding protein C-terminus binding protein domain specific binding identical protein binding protein homodimerization activity metal ion binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding C2H2 zinc finger transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter mesonephros development transcription, DNA-templated apoptotic process central nervous system development negative regulation of cell proliferation embryonic pattern specification anterior/posterior pattern specification protein ubiquitination hemopoiesis myeloid cell differentiation positive regulation of chondrocyte differentiation protein localization to nucleus embryonic hindlimb morphogenesis forelimb morphogenesis embryonic digit morphogenesis positive regulation of apoptotic process positive regulation of fat cell differentiation negative regulation of myeloid cell differentiation positive regulation of ossification negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated male germ-line stem cell asymmetric division positive regulation of NK T cell differentiation cartilage development positive regulation of cartilage development skeletal system development regulation of transcription, DNA-templated transcription from RNA polymerase II promoter embryonic limb morphogenesis hindlimb morphogenesis

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PLZF Monoclonal Antibody (Mags.21F7), Alexa Fluor™ 488, eBioscience™

PLZF Antibody (53-9320-82) in Flow

Product Details

53-9320-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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