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PNK Antibody (PA5-93150) in ICC/IF
Immunofluorescence analysis of PNK in GFP-RNF168 transgenic U2OS cells. Samples were incubated with PNK Polyclonal antibody (Product # PA5-93150). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
产品信息
PA5-93150
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_0091852)
偶联物
形式
Liquid
浓度
0.68 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2806643
产品详细信息
Immunogen sequence: RTVAVKQLGV NPSTTGTQEL KPGLEGSLGV GDTLYLVNGL HPLTLRWEET RTPESQPDTP PGTPLVSQDE KRDAELPKKR MRKSNPGWEN LEKLLVFTAA GVKPQGKVAG FDLDGTLITT RSGKVFPTGP SDWRILYPEI PRKLRELEAE GYKLVIFTNQ MSIGRGKLPA EEFKAKVEAV VEKLGVPFQV LVATHAGLYR KPVTGMWDHL QEQANDGTPI SIGDSIFVGD AAGRPANWAP GRKKKDFSCA DRLFALNLGL P; Positive Samples: HL-60, HepG2, Rat liver; Cellular Location: Nucleus
靶标信息
Anti-human Polynucleotide Kinase Mouse Monoclonal Antibody recognizes polynucleotide kinase (PNK), a 57 kDa nuclear enzyme implicated in DNA strand repair processes that occur during DNA replication and recombination, or in response to DNA damaging agents. PNK targets the DNA 5'-hydroxy and 3'-phosphate termini for phosphorylation and dephosphorylation events. During strand breakage in human cells, normal PNK associates with other nuclear proteins to prepare the DNA termini for the enzymatic actions of DNA polymerase-beta and DNA ligase III. PNK is a key functional component in maintaining the genetic stability of human cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP); phosphatase/kinase; PKNP; Polynucleotide kinase-3'-phosphatase
基因别名: 1810009G08Rik; AOA4; EIEE10; MCSZ; PNK; PNKP
UniProt ID: (Human) Q96T60
Entrez Gene ID: (Human) 11284, (Mouse) 59047, (Rat) 308576
damaged DNA binding
double-stranded DNA binding
endonuclease activity
protein binding
ATP binding
purine nucleotide binding
nucleotide kinase activity
polynucleotide 3'-phosphatase activity
ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity
nucleotide binding
catalytic activity
kinase activity
transferase activity
hydrolase activity
molecular_function
nucleotide-excision repair, DNA damage removal
DNA-dependent DNA replication
DNA repair
response to oxidative stress
response to radiation
dephosphorylation
positive regulation of telomere maintenance via telomerase
DNA damage response, detection of DNA damage
nucleotide phosphorylation
positive regulation of telomerase activity
nucleic acid phosphodiester bond hydrolysis
DNA 3' dephosphorylation involved in DNA repair
polynucleotide 3' dephosphorylation
positive regulation of telomere capping
cellular response to DNA damage stimulus
metabolic process
phosphorylation
biological_process
