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POLL Antibody (PA5-95516) in WB
Western blot analysis of POLL in Lane 1: human HeLa whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with POLL polyclonal antibody (Product # PA5-95516) using a 0.5 µg/mL dilution, followed by a goat anti-ra... View More
Product Details
PA5-95516
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human DNA Polymerase lambda recombinant protein (Position: K307-N407).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2807318
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human COLO-320 whole cell, human MCF-7 whole cell, human HepG2 whole cell. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA polymerase beta-2; DNA polymerase beta-N; DNA polymerase kappa; DNA polymerase lambda; DNA-directed DNA polymerase lambda; EC 2.7.7.7; FLJ46002; OTTHUMP00000020323; Pol beta2; Pol Lambda; POL-KAPPA; polymerase (DNA directed), lambda; RP11-529I10.1
Gene Aliases: 1110003P06Rik; AV007317; BETAN; Polk; POLKAPPA; POLL
UniProt ID: (Human) Q9UGP5, (Mouse) Q9QXE2, (Rat) Q5RKI3
Entrez Gene ID: (Human) 27343, (Mouse) 56626, (Rat) 361767
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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