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POLR1B Antibody (PA5-106654) in ICC/IF
Immunocytochemistry analysis of POLR1B in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with POLR1B polyclonal antibody (Product # PA5-106654) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-106654
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human POLR1B(Accession Q9H9Y6), corresponding to amino acid residues Q681-D731.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2854322
产品详细信息
Antibody detects endogenous levels of total RPA2.
靶标信息
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: A127; DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I 135kDa polypeptide; DNA-directed RNA polymerase I subunit RPA2; polymerase (RNA) I polypeptide B; polymerase (RNA) I polypeptide B, 128kDa; polymerase (RNA) I subunit B; RNA polymerase 1-2; RNA polymerase 1-2, 128 kDa subunit; RNA polymerase I (127 kDa subunit); RNA polymerase I polypeptide B; RNA polymerase I subunit 2; RPA135
基因别名: 128kDa; D630020H17Rik; POLR1B; RPA116; RPA135; RPA2; Rpo1-2
UniProt ID: (Human) Q9H9Y6, (Mouse) P70700, (Rat) O54888
Entrez Gene ID: (Human) 84172, (Mouse) 20017, (Rat) 83582
transcription initiation from RNA polymerase I promoter
transcription elongation from RNA polymerase I promoter
termination of RNA polymerase I transcription
embryo implantation
rRNA transcription
nucleologenesis
positive regulation of gene expression, epigenetic
transcription, DNA-templated
embryo development
transcription from RNA polymerase I promoter
biological_process
