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POLR1B Antibody (PA5-88309) in WB
Western blot analysis of POLR1B in extracts of various cell lines. Samples were incubated with POLR1B Polyclonal antibody (Product # PA5-88309) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
PA5-88309
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 670-900 of human POLR1B (NP_0618872)
偶联物
形式
Liquid
浓度
0.65 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2804819
产品详细信息
Immunogen sequence: IANFIPFSDH NQSPRNMYQC QMGKQTMGFP LLTYQDRSDN KLYRLQTPQS PLVRPSMYDY YDMDNYPIGT NAIVAVISYT GYDMEDAMIV NKASWERGFA HGSVYKSEFI DLSEKIKQGD SSLVFGIKPG DPRVLQKLDD DGLPFIGAKL QYGDPYYSYL NLNTGESFVM YYKSKENCVV DNIKVCSNDT GSGKFKCVCI TMRVPRNPTI GDKFASRHGQ KGILSRLWPA E; Positive Samples: HeLa, HT-29, 293T, Mouse spleen; Cellular Location: Nucleus, nucleolus
靶标信息
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I 135kDa polypeptide; DNA-directed RNA polymerase I subunit RPA2; polymerase (RNA) I polypeptide B; polymerase (RNA) I polypeptide B, 128kDa; polymerase (RNA) I subunit B; RNA polymerase 1-2, 128 kDa subunit; RNA polymerase I subunit 2; RPA135
基因别名: 128kDa; D630020H17Rik; POLR1B; RPA116; RPA135; RPA2; Rpo1-2
UniProt ID: (Human) Q9H9Y6, (Mouse) P70700
Entrez Gene ID: (Human) 84172, (Mouse) 20017
transcription initiation from RNA polymerase I promoter
transcription elongation from RNA polymerase I promoter
termination of RNA polymerase I transcription
embryo implantation
rRNA transcription
nucleologenesis
positive regulation of gene expression, epigenetic
transcription, DNA-templated
embryo development
