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POLR1E Antibody (PA5-116551) in WB
Western blot analysis of POLR1E in extracts of various cell lines. Samples were incubated with POLR1E Polyclonal antibody (Product # PA5-116551) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-116551
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_0719351)
偶联物
形式
Liquid
浓度
1.38 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2901182
产品详细信息
Positive Samples: HeLa, A-549, 293T, Jurkat
Immunogen sequence: MAAEVLPSAR WQYCGAPDGS QRAVLVQFSN GKLQSPGNMR FTLYENKDST NPRKRNQRIL AAETDRLSYV GNNFGTGALK CNTLCRHFVG ILNKTSGQME VYDAELFNMQ PLFSDVSVES ELALESQTKT YREKMDSCIE AFGTTKQKRA LNTRRMNRVG NESLNRAVAK AAETIIDTKG VTALVSDAIH NDLQDDSLYL PPCYDDAAKP EDVYKFEDLL SPAEYEALQS PSEAFRNVTS EEILKMIEEN SHCTFVIEAL KSLPSDVESR DRQARCIWFL DTLIKFRAHR VVKRKSALGP GVPHIINTKL LKHFTCLTYN NGRLRNLISD SMKAKITAYV IILALHIHDF QIDLTVLQRD LKLSEKRMME IAKAMRLKIS KRRVSVAAGS EEDHKLGTLS LPLPPAQTSD RLAKRRKIT
靶标信息
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors (PubMed:24207024). Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (PubMed:24207024). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA-directed RNA polymerase I subunit E; DNA-directed RNA polymerase I subunit RPA49; FLJ13390; FLJ13970; polymerase (RNA) I associated factor 1; polymerase (RNA) I polypeptide E; polymerase (RNA) I polypeptide E, 53kDa; polymerase (RNA) I subunit E; RNA polymerase I associated factor (Paf53); RNA polymerase I subunit A49; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53; RP11-405L18.3
基因别名: 53kDa; AU042259; D030019D19Rik; PAF53; POLR1E; PRAF1; RGD1565773
UniProt ID: (Human) Q9GZS1, (Mouse) Q8K202
Entrez Gene ID: (Human) 64425, (Mouse) 64424, (Rat) 313245
RNA polymerase I transcriptional preinitiation complex assembly at the promoter for the nuclear large rRNA transcript
transcription initiation from RNA polymerase I promoter
transcription elongation from RNA polymerase I promoter
termination of RNA polymerase I transcription
rRNA transcription
positive regulation of gene expression, epigenetic
transcription, DNA-templated
biological_process
