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POLR2C Antibody (PA5-31255) in ICC/IF
POLR2C Polyclonal Antibody detects POLR2C protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: POLR2C protein stained by POLR2C Polyclonal Antibody (Product # PA5-31255) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-31255
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 25 and 241 of Human POLR2C
Conjugate
Form
Liquid
Concentration
0.98 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2548729
Product Specific Information
Recommended positive controls: 293T, A431, Jurkat.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (92%), Xenopus laevis (94%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-directed RNA polymerase II 33 kDa polypeptide; DNA-directed RNA polymerase II subunit C; DNA-directed RNA polymerase II subunit RPB3; polymerase (RNA) II (DNA directed) polypeptide C, 33kDa; polymerase (RNA) II subunit C; polymerase II polypeptide C; RNA polymerase II 3; RNA polymerase II subunit 3; RNA polymerase II subunit B3; RPB31; RPB33
Gene Aliases: 33kDa; A-152E5.7; hRPB33; hsRPB3; mRBP31; POLR2C; RPB3; RPB31; Rpo2-3
UniProt ID: (Human) P19387, (Mouse) P97760
Entrez Gene ID: (Human) 5432, (Rat) 361365, (Mouse) 20021
mRNA splicing, via spliceosome
transcription-coupled nucleotide-excision repair
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
7-methylguanosine mRNA capping
fibroblast growth factor receptor signaling pathway
gene expression
gene silencing by RNA
somatic stem cell population maintenance
snRNA transcription from RNA polymerase II promoter
positive regulation of viral transcription
transcription, DNA-templated
Performance Guarantee
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