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POMGNT1 Antibody (PA5-100281) in ICC/IF
Immunofluorescent analysis of POMGNT1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and POMGNT1 polyclonal antibody (Product # PA5-100281) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100281
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human POMGNT1(Accession Q8WZA1), corresponding to amino acid residues L622-T660.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2815811
Product Specific Information
Antibody detects endogenous levels of total MGAT1.
Target Information
The product of the POMGNT1 gene, protein O-mannose beta-1,2-N-acetylglucosaminyltransferase, participates in O-mannosyl glycan synthesis. POMGnT1 is an N(in)/C(out) (type II) membrane protein localized in the medial-Golgi that initiates the conversion of high mannose N-glycans to complex N-glycans. Specifically, POMGnT1 is a glycosylation enzyme that participates in the synthesis of O-mannosyl glycan, a laminin-binding ligand of alpha-dystroglycan that is rarely synthesized in mammals. Mutations in the POMGNT1 gene cause muscle-eye-brain disease (MEB), an autosomal recessive disorder characterized by congenital muscular dystrophy, ocular abnormalities and lissencephaly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GnT I.2; MDDGA3; MDDGB3; MDDGC3; O-linked mannose beta1,2-N-acetylglucosaminyltransferase; O-mannosyl N-acetylglucosaminyltransferase; OMGnT1; POMGnT1; protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase; Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1; RP11-322N21.3; UDP-GlcNAc:alpha-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I.2
Gene Aliases: 0610016I07Rik; 4930467B06Rik; GnT I.2; gnT-I.2; GNTI.2; LGMD2O; MEB; MGAT1.2; POMGNT1; UNQ746/PRO1475
UniProt ID: (Human) Q8WZA1, (Rat) Q5XIN7, (Mouse) Q91X88
Entrez Gene ID: (Human) 55624, (Rat) 362567, (Mouse) 68273
protein binding
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity
molecular_function
alpha-1,6-mannosyltransferase activity
alpha-1,2-mannosyltransferase activity
mannosyltransferase activity
alpha-1,3-mannosyltransferase activity
alpha-1,3-galactosyltransferase activity
UDP-glucose:glycoprotein glucosyltransferase activity
glycolipid mannosyltransferase activity
oligosaccharyl transferase activity
dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity
acetylglucosaminyltransferase activity
acetylgalactosaminyltransferase activity
galactosyltransferase activity
fucosyltransferase activity
O antigen polymerase activity
lipopolysaccharide-1,6-galactosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
cellulose synthase activity
9-phenanthrol UDP-glucuronosyltransferase activity
1-phenanthrol glycosyltransferase activity
9-phenanthrol glycosyltransferase activity
1,2-dihydroxy-phenanthrene glycosyltransferase activity
phenanthrol glycosyltransferase activity
beta-1,4-mannosyltransferase activity
alpha-1,2-galactosyltransferase activity
dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity
lipopolysaccharide-1,5-galactosyltransferase activity
dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity
dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity
inositol phosphoceramide synthase activity
glucosyltransferase activity
alpha-(1->3)-fucosyltransferase activity
alpha-(1->6)-fucosyltransferase activity
indole-3-butyrate beta-glucosyltransferase activity
salicylic acid glucosyltransferase (ester-forming) activity
salicylic acid glucosyltransferase (glucoside-forming) activity
benzoic acid glucosyltransferase activity
chondroitin hydrolase activity
dolichyl-pyrophosphate Man7GlcNAc2 alpha-1,6-mannosyltransferase activity
cytokinin 9-beta-glucosyltransferase activity
protein modifying enzyme
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