Invitrogen

PP1 alpha Monoclonal Antibody (10C6-3)

PP1 alpha Antibody in Immunocytochemistry (ICC/IF)

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PP1 alpha Antibody (43-8100) in ICC/IF

Immunofluorescence analysis of PP1 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PP1 (10C6-3) Mouse Monoclonal Antibody (Product # 43-8100) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room tem... View More

PP1 alpha Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

43-8100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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免疫细胞化学 (ICC/IF)

2 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-dependent

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Dog, Human, Mouse, Non-human primate, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

10C6-3

抗原

Mixture of two peptides from the C-terminal regions of human PP1 alpha protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2533541

靶标信息

This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Dietary potassium stimulates Ppp1Ca-Ppp1r1a dephosphorylation of kidney NaCl cotransporter and reduces blood pressure.

The Journal of clinical investigation

Grimm PR,Tatomir A,Rosenbaek LL,Kim BY,Li D,Delpire EJ,Fenton RA,Welling PA

43-8100 was used in Immunoprecipitation to confirm that PP1A dephosphorylated NCC directly in a potassium-regulated manner using mice genetically engineered to constitutively activate the NCC-regulatory kinase SPAK and thereby eliminate the effects of the WNK/SPAK kinase cascade.

Wed Nov 01 00:00:00 UTC 2023

Dietary potassium stimulates Ppp1Ca-Ppp1r1a dephosphorylation of kidney NaCl cotransporter and reduces blood pressure.

The Journal of clinical investigation

Grimm PR,Tatomir A,Rosenbaek LL,Kim BY,Li D,Delpire EJ,Fenton RA,Welling PA

Wed Nov 01 00:00:00 UTC 2023

Deficiency of macrophage PHACTR1 impairs efferocytosis and promotes atherosclerotic plaque necrosis.

The Journal of clinical investigation

Kasikara C,Schilperoort M,Gerlach B,Xue C,Wang X,Zheng Z,Kuriakose G,Dorweiler B,Zhang H,Fredman G,Saleheen D,Reilly MP,Tabas I

43-8100 was used in Immunoprecipitation to study whether lower expression of PHACTR1 in macrophages in subjects carrying the rs9349379-G allele contributes to atherosclerosis by compromising efferocytosis, specifically the ability of macrophages to internalize bound apoptotic cells.

Thu Apr 15 00:00:00 UTC 2021

Deregulated PP1α phosphatase activity towards MAPK activation is antagonized by a tumor suppressive failsafe mechanism.

Nature communications

Chen M,Wan L,Zhang J,Zhang J,Mendez L,Clohessy JG,Berry K,Victor J,Yin Q,Zhu Y,Wei W,Pandolfi PP

43-8100 was used in Immunoprecipitation to reveal a PP1α/PML molecular network that is genetically altered in human cancer towards aberrant MAPK activation, with important therapeutic implications.

Mon Jan 15 00:00:00 UTC 2018

Deregulated PP1α phosphatase activity towards MAPK activation is antagonized by a tumor suppressive failsafe mechanism.

Nature communications

Chen M,Wan L,Zhang J,Zhang J,Mendez L,Clohessy JG,Berry K,Victor J,Yin Q,Zhu Y,Wei W,Pandolfi PP

43-8100 was used in Immunoprecipitation to reveal a PP1α/PML molecular network that is genetically altered in human cancer towards aberrant MAPK activation, with important therapeutic implications.

Mon Jan 15 00:00:00 UTC 2018

MYC dephosphorylation by the PP1/PNUTS phosphatase complex regulates chromatin binding and protein stability.

Nature communications

Dingar D,Tu WB,Resetca D,Lourenco C,Tamachi A,De Melo J,Houlahan KE,Kalkat M,Chan PK,Boutros PC,Raught B,Penn LZ

43-8100 was used in Western Blotting to identify the regulatory mechanisms controlling c-MYC oncoprotein.

Wed Aug 29 00:00:00 UTC 2018

PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications.

Cell cycle (Georgetown, Tex.)

Kourtidis A,Anastasiadis PZ

43-8100 was used in immunocytochemistry and western blot to characterize an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications defined by PLEKHA7

Tue Dec 13 00:00:00 UTC 2016

PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications.

Cell cycle (Georgetown, Tex.)

Kourtidis A,Anastasiadis PZ

43-8100 was used in immunocytochemistry and western blot to characterize an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications defined by PLEKHA7

Tue Dec 13 00:00:00 UTC 2016

lncRNA directs cooperative epigenetic regulation downstream of chemokine signals.

Cell

Xing Z,Lin A,Li C,Liang K,Wang S,Liu Y,Park PK,Qin L,Wei Y,Hawke DH,Hung MC,Lin C,Yang L

43-8100 was used in western blot to elucidate the contribution of lncRNA BCAR4 in breast cancer metastasis.

Thu Nov 20 00:00:00 UTC 2014

生物信息学

蛋白别名: GADD34; MGC15877; MGC1674; PP-1A; protein phosphatase 1, catalytic subunit, alpha isoform; protein phosphatase 1, catalytic subunit, alpha isozyme; Protein phosphatase type 1 alpha, catalytic subunit; protein phosphatase-1d; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; testicular tissue protein Li 155

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基因别名: dism2; PP-1A; PP1A; PP1alpha; PPP1A; Ppp1c; PPP1CA

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UniProt ID: (Human) P62136, (Mouse) P62137, (Rat) P62138

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Entrez Gene ID: (Human) 5499, (Dog) 403609, (Mouse) 19045, (Rat) 24668

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