Invitrogen

PPM1A Recombinant Rabbit Monoclonal Antibody (006)

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PPM1A Antibody (MA5-29750) in WB

Western Blot was performed using Anti-PP2CA Recombinant Rabbit Monoclonal Antibody (Product # MA5-29750) and a 42 kDa band corresponding to Protein phosphatase 1A was observed across cell lines and tissues. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), THP-1 (Lane 2), HeLa (Lane 3), Mouse Brain (Lane 4), Rat Brain (Lane 5), Mouse Kidney (Lane 6), Rat Kidney (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB... View More

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PPM1A Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Protein phosphatase 1A siRNA and decrease in signal intensity was observed in Western Blot application using Anti-PP2CA Recombinant Rabbit Monoclonal Antibody (6) (Product # MA5-29750). {KD}

产品信息

MA5-29750

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

006

抗原

Recombinant Mouse PPM1A/PP2CA Protein (Met1-Trp382)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2785573

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Mouse PPM1A/PP2CA.

靶标信息

PPM1A (Protein phosphatase 1A) is also named as PPPM1A and belongs to the PP2C family. It is a serine/threonine protein phosphatase that is essential for regulating cellular stress responses in eukaryotes. PPM1A plays an important role in tumor suppression and that it negatively controls many signaling pathways which are critical for trophoblast invasion. This protein distributes in both the cytoplasm and nucleus of mammalian cells. It has 2 isoforms produced by alternative splicing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: IA; Mpp alpha; PP2C-alpha; Protein phosphatase 1A; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform; protein phosphatase 1A, magnesium dependent, alpha isoform; Protein phosphatase 2C isoform alpha; Protein phosphatase IA; Protein phosphatase type 1A (formely 2C) Mg-dependent alpha isoform; Protein phosphatase type 1A (formely 2C), Mg-dependent, alpha isoform; protein phosphatase, Mg2+/Mn2+ dependent, 1A

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基因别名: 2310003C21Rik; 2900017D14Rik; AI427932; AU017636; MMPa-2; MPPa-1; PP2C-ALPHA; Pp2c1; PP2CA; PP2Calpha; PPM1A; PPPM1A

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UniProt ID: (Human) P35813, (Mouse) P49443, (Rat) P20650

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Entrez Gene ID: (Human) 5494, (Mouse) 19042, (Rat) 24666

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