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PPP2R4 Antibody (PA5-29755) in ICC/IF
Immunofluorescence analysis of PPP2R4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PPP2R4 Rabbit Polyclonal Antibody (Product # PA5-29755) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A... View More
产品信息
PA5-29755
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein fragment corresponding to a region within amino acids 1 and 159 of Human PPP2R4
偶联物
形式
Liquid
浓度
0.74 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2547229
产品详细信息
Recommended positive controls: A549, HeLa, HepG2, HCT116.
Predicted reactivity: Mouse (96%), Rat (95%), Rabbit (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: MGC2184; OTTHUMP00000022333; Phosphotyrosyl phosphatase activator; PP2A phosphatase activator; PP2A subunit B' isoform PR53; PP2A, subunit B', PR53 isoform; protein phosphatase 2 regulatory subunit 4; protein phosphatase 2A activator, regulatory subunit 4; protein phosphatase 2A regulatory subunit 4; protein phosphatase 2A, regulatory subunit B' (PR 53); PTPA; Serine/threonine-protein phosphatase 2A activator; Serine/threonine-protein phosphatase 2A regulatory subunit 4; Serine/threonine-protein phosphatase 2A regulatory subunit B'
基因别名: PP2A; PPP2R4; PR53; PTPA
UniProt ID: (Human) Q15257
Entrez Gene ID: (Human) 5524
peptidyl-prolyl cis-trans isomerase activity
receptor binding
protein binding
ATP binding
protein tyrosine phosphatase activator activity
protein phosphatase type 2A regulator activity
ATPase activity
protein homodimerization activity
protein heterodimerization activity
protein phosphatase 2A binding
protein-binding activity modulator
phosphatase activator
phosphatase modulator
protein peptidyl-prolyl isomerization
mitotic spindle organization in nucleus
negative regulation of phosphoprotein phosphatase activity
positive regulation of phosphoprotein phosphatase activity
regulation of protein phosphatase type 2A activity
positive regulation of protein dephosphorylation
negative regulation of protein dephosphorylation
positive regulation of apoptotic process
regulation of phosphoprotein phosphatase activity
