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PPT1 Antibody (PA5-102896) in ICC/IF
Immunofluorescent analysis of PPT1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and PPT1 polyclonal antibody (Product # PA5-102896) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-102896
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PPT1(Accession P50897), corresponding to amino acid residues S130-L180.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852277
Product Specific Information
Antibody detects endogenous levels of total PPT1.
Target Information
The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3.1.2.22; ceroid-palmitoyl-palmitoyl-protein thioesterase 1; Palmitoyl-protein hydrolase 1; Palmitoyl-protein thioesterase 1; PPT-1
Gene Aliases: 9530043G02Rik; AA960502; C77813; CLN1; D4Ertd184e; INCL; PPT; PPT1
UniProt ID: (Human) P50897, (Rat) P45479, (Mouse) O88531
Entrez Gene ID: (Human) 5538, (Rat) 29411, (Mouse) 19063
protein depalmitoylation
receptor-mediated endocytosis
pinocytosis
lysosomal lumen acidification
chemical synaptic transmission
neurotransmitter secretion
nervous system development
brain development
visual perception
grooming behavior
associative learning
adult locomotory behavior
protein transport
lipid catabolic process
sphingolipid catabolic process
protein catabolic process
negative regulation of cell growth
membrane raft organization
regulation of phospholipase A2 activity
long-chain fatty-acyl-CoA biosynthetic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
cellular protein catabolic process
positive regulation of receptor-mediated endocytosis
positive regulation of pinocytosis
neuron development
regulation of synapse structure or activity
response to stimulus
cofactor transport
cofactor metabolic process
lysosome organization
cellular macromolecule catabolic process
regulation of synaptic plasticity
macromolecule depalmitoylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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