Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 5
PPT1 Antibody (PA5-29177) in IHC (P)
PPT1 Polyclonal Antibody detects PPT1 protein at lysosome on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. PPT1 Polyclonal Antibody (Product # PA5-29177) dilution: 1:500. Scale bar = M_2 µm. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-29177
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 306 of Human PPT1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546653
Product Specific Information
Recommended positive controls: 293T, Raji.
Predicted reactivity: Mouse (88%), Rat (87%), Dog (94%), Rhesus Monkey (99%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 3.1.2.22; ceroid-palmitoyl-palmitoyl-protein thioesterase 1; Palmitoyl-protein hydrolase 1; Palmitoyl-protein thioesterase 1; PPT-1
Gene Aliases: 9530043G02Rik; AA960502; C77813; CLN1; D4Ertd184e; INCL; PPT; PPT1
UniProt ID: (Human) P50897, (Rat) P45479, (Mouse) O88531
Entrez Gene ID: (Human) 5538, (Rat) 29411, (Mouse) 19063
protein depalmitoylation
receptor-mediated endocytosis
pinocytosis
lysosomal lumen acidification
chemical synaptic transmission
neurotransmitter secretion
nervous system development
brain development
visual perception
grooming behavior
associative learning
adult locomotory behavior
protein transport
lipid catabolic process
sphingolipid catabolic process
protein catabolic process
negative regulation of cell growth
membrane raft organization
regulation of phospholipase A2 activity
long-chain fatty-acyl-CoA biosynthetic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
cellular protein catabolic process
positive regulation of receptor-mediated endocytosis
positive regulation of pinocytosis
neuron development
regulation of synapse structure or activity
response to stimulus
cofactor transport
cofactor metabolic process
lysosome organization
cellular macromolecule catabolic process
regulation of synaptic plasticity
macromolecule depalmitoylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support