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PRDM16 Antibody (720206) in WB
Western blot analysis was performed on Whole cell extracts (30 µg lysate) of 3T3L1 (Lane 1), LNCap (Lane 2) and tissue extracts of Rat Adipose (Lane 3) and Mouse skin (Lane 4). The blots were probed with Anti-PRDM16/MEL1 Rabbit Polyclonal Antibody (Product # 720206, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Two isoforms of 140 and 120 kDa corresponding to PRDM16/MEL1 was observed. Known quantity of prote... View More
产品信息
720206
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptides corresponding to human PRDM16/MEL1 (236-251, 23-40)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 30% glycerol, 0.1% BSA
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2664823
产品详细信息
This antibody is predicted to react with Monkey, Mouse, Dog
靶标信息
PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36, q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Histone-lysine N-methyltransferase PRDM16; line 27; MDS1/EVI1-like gene 1; PR domain containing 16; PR domain zinc finger protein 16; PR domain-containing protein 16; Transcription factor MEL1
基因别名: 5730557K01Rik; CMD1LL; csp1; KIAA1675; LVNC8; MEL1; PFM13; PRDM16
UniProt ID: (Human) Q9HAZ2, (Mouse) A2A935
Entrez Gene ID: (Human) 63976, (Rat) 100366024, (Mouse) 70673
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
neurogenesis
negative regulation of transforming growth factor beta receptor signaling pathway
histone lysine methylation
somatic stem cell population maintenance
regulation of cellular respiration
tongue development
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
white fat cell differentiation
brown fat cell differentiation
palate development
positive regulation of brown fat cell differentiation
regulation of transcription, DNA-templated
biological_process
stem cell population maintenance
negative regulation of granulocyte differentiation
cell differentiation
