Invitrogen

PRDX1 Polyclonal Antibody

PRDX1 Antibody in Immunocytochemistry (ICC/IF)

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PRDX1 Antibody (PA5-27487) in ICC/IF

Immunofluorescence analysis of PRDX1 was performed using 70% confluent log phase HeLa treated with Hydrogen peroxide (0.5mM, 2 hrs). The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PRDX1 Polyclonal Antibody (Product # PA5-27487) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa F... View More

PRDX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PRDX1 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using PRDX1 Polyclonal Antibody(Product #PA5-27487), shows upregulation upon Hydrogen peroxide treatment in HeLa and MCF7. {TM}

产品信息

PA5-27487

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 146 of Human PRX I

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.22 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2544963

产品详细信息

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3.

Predicted reactivity: Mouse (95%), Rat (98%), Zebrafish (80%), Dog (97%), Chicken (86%), Rhesus Monkey (98%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: ACR1; Alu co-repressor 1; AOEB166; B166; HBP23; Heme-binding 23 kDa protein; macrophage 23 Kd stress protein; Macrophage 23 kDa stress protein; macrophage 23kDa stress protein; macrophase stress protein 22kDa; macrophase stress protein 23 kd; Natural killer cell-enhancing factor A; natural killer-enhancing factor A; NKEF-A; OSF-3; osteoblast specific factor 3; Osteoblast-specific factor 3; PAG; Peroxiredoxin-1; proliferation-associated gene A; Proliferation-associated gene protein; Prx; Prx 1; thioredoxin dependent peroxide reductase 2; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2; Thioredoxin-dependent peroxiredoxin 1; Trx dependent peroxide reductase 2

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基因别名: Hbp23; MSP23; NKEF-A; NKEFA; OSF-3; OSF3; PAG; PAGA; PAGB; PRDX1; PrdxI; PRX1; PRXI; TDPX2; TDX2; TPxA

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UniProt ID: (Human) Q06830, (Mouse) P35700, (Rat) Q63716

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Entrez Gene ID: (Human) 5052, (Mouse) 18477, (Rat) 117254

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功能