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PRELP Antibody (PA5-47841) in ICC/IF
Immunocytochemistry analysis of PRELP in immersion fixed human mesenchymal stem cells differentiated into chondrocytes. Samples were incubated in PRELP polyclonal antibody (Product # PA5-47841) using a dilution of 10 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
PA5-47841
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CHO-derived recombinant human PRELP Gln21-Ile382
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608591
Product Specific Information
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
PRELP is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 55 kDa leucine-rich repeat protein of articular cartilage; Prolargin; prolargin proteoglycan; Proline-arginine-rich end leucine-rich repeat protein; proline/arginine-rich end leucine-rich repeat protein
Gene Aliases: MST161; MSTP161; PRELP; SLRR2A
UniProt ID: (Human) P51888
Entrez Gene ID: (Human) 5549
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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