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PRKAR2A Antibody (PA5-106470) in ICC/IF
Immunocytochemistry analysis of PRKAR2A in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PRKAR2A polyclonal antibody (Product # PA5-106470) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-106470
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PRKAR2A(Accession P13861), corresponding to amino acid residues S48-V98.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2854139
产品详细信息
Antibody detects endogenous levels of total PKA R2.
靶标信息
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of AMPK.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cAMP-dependent protein kinase regulatory subunit RII alpha; cAMP-dependent protein kinase type II-alpha regulatory subunit; MGC3606; protein kinase A, RII-alpha subunit; protein kinase cAMP-dependent regulatory type II alpha; protein kinase, cAMP-dependent, regulatory subunit type II alpha; protein kinase, cAMP-dependent, regulatory, type 2, alpha
基因别名: 1110061A24Rik; AI317181; AI836829; PKR2; PRKAR2; PRKAR2A; RII(alpha)
UniProt ID: (Human) P13861, (Rat) P12368
Entrez Gene ID: (Human) 5576, (Mouse) 19087, (Rat) 29699
cAMP-dependent protein kinase inhibitor activity
protein binding
cAMP-dependent protein kinase regulator activity
cAMP binding
ubiquitin protein ligase binding
protein kinase A catalytic subunit binding
nucleotide binding
protein domain specific binding
small molecule binding
protein homodimerization activity
kinase activity
protein-binding activity modulator
kinase modulator
renal water homeostasis
blood coagulation
activation of protein kinase A activity
intracellular signal transduction
cellular response to glucagon stimulus
negative regulation of cAMP-dependent protein kinase activity
regulation of protein phosphorylation
regulation of protein kinase activity
protein heterooligomerization
phosphorylation
response to clozapine
