Invitrogen

PRKD2 Polyclonal Antibody

查看其他21个PRKD2抗体

抗体检测数据 (4)

PRKD2 Antibody in Immunocytochemistry (ICC/IF)

PRKD2 Antibody in Immunocytochemistry (ICC/IF)

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PRKD2 Antibody (PA5-21427) in ICC/IF

PRKD2 Polyclonal Antibody detects PKD2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PKD2 protein stained by PRKD2 Polyclonal Antibody (Product # PA5-21427) diluted at 1:200. Blue: Hoechst 33342 staining.

PRKD2 Antibody in Immunocytochemistry (ICC/IF)

PRKD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRKD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRKD2 Antibody in Western Blot (WB)

Product Image Gallery

PRKD2 Antibody in Immunocytochemistry (ICC/IF) — PRKD2 Polyclonal Antibody detects PKD2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PKD2 protein stained by PRKD2 Polyclonal Antibody (Product # PA5-21427) diluted at 1:200. Blue: Hoechst 33342 staining.

PRKD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — PRKD2 Polyclonal Antibody detects PKD2 protein at cytosol and membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. PRKD2 Polyclonal Antibody (Product # PA5-21427) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PRKD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — PRKD2 Polyclonal Antibody detects PKD2 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PRKD2 Polyclonal Antibody (Product # PA5-21427) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PRKD2 Antibody in Western Blot (WB) — Western Blot analysis of Protein Kinase D2 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Protein Kinase D2 Polyclonal Antibody (Product # PA5-21427) at a dilution of 1:500.

产品信息

PA5-21427

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

-

免疫细胞化学 (ICC/IF)

1:100-1:1,000

-

免疫沉淀 (IP)

Assay-dependent

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 529 and 862 of PKD2 (Uniprot ID#Q9BZL6)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.99 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11152504

产品详细信息

Recommended positive controls: Molt4.

Predicted reactivity: Mouse (99%), Rat (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

PRKD2 (PKD2) is a member of the protein kinase D serine/threonine protein kinase family. PRKD2 is a component in the signal transduction pathway induced by phorbol esters and the CCK b gastrin receptor. It exhibits the strongest homology to the serine threonine protein kinases PKD/PKC and PKCnu, particularly in the duplex zinc finger-like cysteine-rich motif, in the pleckstrin homology domain and in the protein kinase domain. In contrast, it shows only a low degree of sequence similarity to other members of the PKC family.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: nPKC-D2; Serine/threonine-protein kinase D2

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基因别名: AI325941; HSPC187; nPKC-D2; PKD2; PRKD2

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UniProt ID: (Human) Q9BZL6, (Mouse) Q8BZ03

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Entrez Gene ID: (Human) 25865, (Mouse) 101540

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protein kinase activity protein serine/threonine kinase activity protein kinase C activity protein binding ATP binding metal ion binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

angiogenesis positive regulation of endothelial cell proliferation adaptive immune response protein phosphorylation cell adhesion cell death positive regulation of endothelial cell migration peptidyl-serine phosphorylation positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of interleukin-2 production positive regulation of interleukin-8 production positive regulation of CREB transcription factor activity positive regulation of peptidyl-serine phosphorylation intracellular signal transduction cellular response to vascular endothelial growth factor stimulus positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway positive regulation of blood vessel endothelial cell migration positive regulation of fibroblast growth factor receptor signaling pathway positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation vascular endothelial growth factor receptor signaling pathway T cell receptor signaling pathway positive regulation of T cell receptor signaling pathway positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity endothelial tube morphogenesis positive regulation of ERK1 and ERK2 cascade protein kinase D signaling mitophagy in response to mitochondrial depolarization positive regulation of histone deacetylase activity positive regulation of intracellular signal transduction positive regulation of DNA biosynthetic process positive regulation of endothelial cell chemotaxis immune system process phosphorylation