Invitrogen

PRMT3 Polyclonal Antibody

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抗体检测数据 (5)

PRMT3 Antibody in Immunocytochemistry (ICC/IF)

PRMT3 Antibody in Immunocytochemistry (ICC/IF)

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PRMT3 Antibody (PA5-115795) in ICC/IF

Immunocytochemistry analysis of PRMT3 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115795). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

PRMT3 Antibody in Immunocytochemistry (ICC/IF)

PRMT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT3 Antibody in Western Blot (WB)

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PRMT3 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of PRMT3 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115795). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

PRMT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PRMT3 in human colorectal cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115795) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

PRMT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PRMT3 in mouse stomach tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115795) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

PRMT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PRMT3 in mouse brain tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115795) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

PRMT3 Antibody in Western Blot (WB) — Western blot analysis of PRMT3 in HCT116 (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115795).

产品信息

PA5-115795

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human PRMT3(Accession O60678), corresponding to amino acid residues M331-A381.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2900430

产品详细信息

Antibody detects endogenous levels of total PRMT3.

靶标信息

Type I protein arginine N-methyltransferases (PRMTs), such as PRMT3, catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: heterogeneous nuclear ribonucleoprotein methyltransferase-like 3; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3; HMT1 hnRNP methyltransferase-like 3; protein arginine methyltransferase 3; Protein arginine N-methyltransferase 3; protein arginine N-methyltransferase 3(hnRNP methyltransferase S. cerevisiae)-like 3

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基因别名: 2010005E20Rik; 2410018A17Rik; AL033309; HRMT1L3; PRMT3

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UniProt ID: (Human) O60678, (Rat) O70467, (Mouse) Q922H1

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Entrez Gene ID: (Human) 10196, (Rat) 89820, (Mouse) 71974

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protein binding methyltransferase activity histone-arginine N-methyltransferase activity protein-arginine N-methyltransferase activity protein-arginine omega-N asymmetric methyltransferase activity metal ion binding S-adenosylmethionine-dependent methyltransferase activity modified amino acid binding rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity protein modifying enzyme zinc finger transcription factor

regulation of transcription, DNA-templated peptidyl-arginine methylation, to asymmetrical-dimethyl arginine negative regulation of protein ubiquitination histone arginine methylation protein methylation peptidyl-arginine methylation dendritic spine morphogenesis methylation peptidyl-arginine N-methylation