Invitrogen

PRMT4 Polyclonal Antibody

PRMT4 Antibody in Immunocytochemistry (ICC/IF)

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PRMT4 Antibody (PA5-81150) in ICC/IF

Immunofluorescence staining of PRMT4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PRMT4 Polyclonal Antibody (Product # PA5-81150, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.

PRMT4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-81150

应用

建议稀释比

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免疫组化（石蜡） (IHC (P))

1:1,000-1:4,000

免疫细胞化学 (ICC/IF)

1:300-1:10,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to the N-terminus of the Human CARM1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2788387

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human CARM1.

靶标信息

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。