Invitrogen

PRMT4 Recombinant Superclonal™ Antibody (2HCLC)

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PRMT4 Antibody (711526) in WB

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), HCT 116 (Lane 4) and HT-29 (Lane 5). The blot was probed with Anti-PRMT4 Recombinant Rabbit Superclonal™ Antibody (Product # 711526, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 65 kDa band corresponding to CARM1 was observed across the cell lines tested.

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PRMT4 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Anti-PRMT4 Recombinant Rabbit Superclonal™ Antibody (Product # 711526, 1:200 dilution), shows increased expression of the protein CARM1 in Hep G2 cells in high-glucose and glucose starvation conditions (PMID: 27309807; 2507291). {TM}

产品信息

711526

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

克隆号

2HCLC

抗原

Protein corresponding to human PRMT4 [aa220-aa482]

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2723513

产品详细信息

This antibody is predicted to react with Monkey, Pig, Mouse, Rat.

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

靶标信息

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。