Invitrogen

PRMT6 Recombinant Rabbit Monoclonal Antibody (JE36-57)

查看其他21个PRMT6抗体

抗体检测数据 (1)

PRMT6 Antibody in Western Blot (WB)

PRMT6 Antibody in Western Blot (WB)

图: 1 / 1

PRMT6 Antibody (MA5-50821) in WB

Western blot analysis of PRMT6 in different various lysates. Lane 1: Human kidney tissue lysate (20 µg/Lane); Lane 2: Mouse kidney tissue lysate (20 µg/Lane); Lane 3: Mouse testis tissue lysate (20 µg/Lane); Lane 4: Rat kidney tissue lysate (20 µg/Lane); Lane 5: Rat testis tissue lysate (20 µg/Lane); Lane 6: 293T cell lysate (10 µg/Lane); Lane 7: HEK-293 cell lysate (10 µg/Lane). Predicted band size: 42 kDa. Observed band size: 42 kDa (Unknown band detected in mouse tissue)Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Primary antibody PRMT... View More

PRMT6 Antibody in Western Blot (WB)

Product Image Gallery

PRMT6 Antibody in Western Blot (WB) — Western blot analysis of PRMT6 in different various lysates. Lane 1: Human kidney tissue lysate (20 µg/Lane); Lane 2: Mouse kidney tissue lysate (20 µg/Lane); Lane 3: Mouse testis tissue lysate (20 µg/Lane); Lane 4: Rat kidney tissue lysate (20 µg/Lane); Lane 5: Rat testis tissue lysate (20 µg/Lane); Lane 6: 293T cell lysate (10 µg/Lane); Lane 7: HEK-293 cell lysate (10 µg/Lane). Predicted band size: 42 kDa. Observed band size: 42 kDa (Unknown band detected in mouse tissue)Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Primary antibody PRMT6 recombinant monoclonal antibody (Product # MA5-50821) with a dilution of 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1:200,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.

产品信息

MA5-50821

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE36-57

抗原

Synthetic peptide within Human PRMT6 aa 191-240/375.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3093860

产品详细信息

Positive control: Human kidney tissue lysate, mouse kidney tissue lysate, mouse testis tissue lysate, rat kidney tissue lysate, rat testis tissue lysate, 293T cell lysate, HEK-293 cell lysate.

Predicted band size: 42 kDa

Subcellular Location: Nucleus.

靶标信息

Protein arginine N-methyltransferase 6 (PRMT6) PRMT6 demonstrates type I PRMT activity, capable of forming both omega-N monomethylarginine and asymmetric dimethylarginine derivatives on the recombinant glycine- and arginine-rich substrates. cts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: FLJ10559; FLJ51477; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; Histone-arginine N-methyltransferase PRMT6; HMT1 hnRNP methyltransferase-like 6; PRMT-6; Protein arginine N-methyltransferase 6

查看更多收起

基因别名: AW124876; BB233495; HRMT1L6; PRMT6

查看更多收起

UniProt ID: (Human) Q96LA8, (Mouse) Q6NZB1

查看更多收起

Entrez Gene ID: (Human) 55170, (Rat) 295384, (Mouse) 99890

查看更多收起

chromatin binding protein binding histone-arginine N-methyltransferase activity protein-arginine N-methyltransferase activity protein-arginine omega-N monomethyltransferase activity protein-arginine omega-N asymmetric methyltransferase activity histone methyltransferase activity histone binding histone methyltransferase activity (H4-R3 specific) histone methyltransferase activity (H3-R2 specific) histone methyltransferase activity (H2A-R3 specific) molecular_function rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity protein modifying enzyme

negative regulation of transcription from RNA polymerase II promoter base-excision repair transcription, DNA-templated viral process cell growth histone methylation peptidyl-arginine methylation, to asymmetrical-dimethyl arginine histone H3-R2 methylation histone H4-R3 methylation negative regulation of transcription, DNA-templated cellular senescence regulation of transcription, DNA-templated biological_process histone arginine methylation DNA repair protein methylation cellular response to DNA damage stimulus chromatin modification methylation peptidyl-arginine N-methylation