Invitrogen

PRPS2 Polyclonal Antibody

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PRPS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRPS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PRPS2 Antibody (PA5-42007) in IHC (P)

Immunohistochemistry (paraffin-embedded) analysis of human kidney tissue using an anti-PRPS2 polyclonal antibody (Product # PA5-42007).

PRPS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRPS2 Antibody in Western Blot (WB)

PRPS2 Antibody in Immunocytochemistry (ICC/IF)

PRPS2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

PRPS2 Antibody in Western Blot (WB)

Product Image Gallery

PRPS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (paraffin-embedded) analysis of human kidney tissue using an anti-PRPS2 polyclonal antibody (Product # PA5-42007).

PRPS2 Antibody in Western Blot (WB) — Western blot analysis of human HepG2 cell lysate using an anti-PRPS2 polyclonal antibody (Product # PA5-42007).

PRPS2 Antibody in Immunocytochemistry (ICC/IF) — Figure 6 PRPS2 targetes E2F1 and activation of the P53/Bcl-xl/Bcl-2 signal pathway. ( a ) Colocation of PRPS2 and E2F1 in GC1 and GC2 cells and normal testis tissue was observed by double immunofluorescent staining. ( b ) After transfection, the expression levels of PRPS2 and E2F1 were detected by double immunofluorescent staining. ( c ) Western blot was used to measure the expression levels of the E2F1/P53/Bcl-xl/Bcl-2 signaling pathway. Values beneath the blots are relative to the control. White arrow: colocation. PRPS2: phosphoribosyl-pyrophosphate synthetase 2; NC: negative control; E2F1: E2F transcription factor 1.

PRPS2 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 2 Correlation of PRPS2 expression with hypospermatogenesis. ( a ) H and E staining of the testis with normal spermatogenesis. ( b ) H and E staining of normal cauda epididymidis. ( c ) H and E staining of a testis with hypospermatogenesis. ( d ) H and E staining of cauda epididymidis in hypospermatogenesis. ( e ) PRPS2 expression was detected in normal testis by IHC. ( f ) PRPS2 expression was detected in a testis with hypospermatogenesis by IHC. Scale bars = 50 mum. ( g ) PRPS2 expression was detected in testis with normal spermatogenesis and hypospermatogenesis by Western blot. ( h ) PRPS2 expression was detected in testis tissues with normal spermatogenesis and hypospermatogenesis by qRT-PCR. *Hypospermatogenesis group compared with normal control, P < 0.05 by independent-samples t -test. Red arrow: spermatogonia; Black arrow: spermatocyte. PRPS2: phosphoribosyl-pyrophosphate synthetase 2; H and E: hematoxylinand and eosin; IHC: immunohistochemical; qRT-PCR: quantitative real-time polymerase chain reaction.

PRPS2 Antibody in Western Blot (WB) — Figure 1 PRPS2 depletion and apoptosis of spermatogenic cells. ( a ) qRT-PCR and Western blot analysis confirmed that PRPS2 was successfully downregulated by gene silencing and upregulated by PRPRS2 overexpression in both GC1 and GC2 cells. ( b ) Flow cytometry analysis shows that PRPS2 depletion promotes the apoptosis of GC1 cells. ( c ) Flow cytometry analysis shows that PRPS2 depletion promotes the apoptosis of GC2 cells (abscissa: cell count; ordinate: the fluorescence intensity). ( d ) Western blot analysis indicates that PRPS2 depletion activates the expression of apoptotic proteins (values beneath blots are relative to the control). *Significantly different compared with negative control, P < 0.05 by independent-samples t -test. shPRPS2: small-hairpin RNA gene silencer; NC: negative control; PRPS2: overexpression; PRPS2: phosphoribosyl-pyrophosphate synthetase 2; qRT-PCR: quantitative real-time polymerase chain reaction; APC-A: Allophycocyanin-A.

产品信息

PA5-42007

应用

建议稀释比

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免疫印迹 (WB)

0.2-1 µg/mL

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免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

4-8 µg/mL

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免疫细胞化学 (ICC/IF)

-

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产品规格

种属反应

Human

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide directed towards the middle region of human PRPS2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2609479

产品详细信息

Peptide sequence: VSPDAGGAKR VTSIADRLNV EFALIHKERK KANEVDRMVL VGDVKDRVAI

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%

靶标信息

The function remains unknown.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis.

Asian journal of andrology

Lei B,Xie LX,Zhang SB,Wan B,Zhong LR,Zhou XM,Mao XM,Shu FP

PA5-42007 was used in Immunohistochemistry (Paraffin) to investigate the relationship of PRPS2 with hypospermatogenesis and spermatogenic cell apoptosis.

Tue Aug 03 00:00:00 UTC 2021

Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis.

Asian journal of andrology

Lei B,Xie LX,Zhang SB,Wan B,Zhong LR,Zhou XM,Mao XM,Shu FP

PA5-42007 was used in Immunohistochemistry (Paraffin) to investigate the relationship of PRPS2 with hypospermatogenesis and spermatogenic cell apoptosis.

Tue Aug 03 00:00:00 UTC 2021

Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis.

Asian journal of andrology

Lei B,Xie LX,Zhang SB,Wan B,Zhong LR,Zhou XM,Mao XM,Shu FP

PA5-42007 was used in Immunohistochemistry (Paraffin) to investigate the relationship of PRPS2 with hypospermatogenesis and spermatogenic cell apoptosis.

Tue Aug 03 00:00:00 UTC 2021

Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis.

Asian journal of andrology

Lei B,Xie LX,Zhang SB,Wan B,Zhong LR,Zhou XM,Mao XM,Shu FP

PA5-42007 was used in Immunohistochemistry (Paraffin) to investigate the relationship of PRPS2 with hypospermatogenesis and spermatogenic cell apoptosis.

Tue Aug 03 00:00:00 UTC 2021

Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis.

Asian journal of andrology

Lei B,Xie LX,Zhang SB,Wan B,Zhong LR,Zhou XM,Mao XM,Shu FP

PA5-42007 was used in Immunohistochemistry (Paraffin) to investigate the relationship of PRPS2 with hypospermatogenesis and spermatogenic cell apoptosis.

Tue Aug 03 00:00:00 UTC 2021

Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis.

Asian journal of andrology

Lei B,Xie LX,Zhang SB,Wan B,Zhong LR,Zhou XM,Mao XM,Shu FP

PA5-42007 was used in Immunohistochemistry (Paraffin) to investigate the relationship of PRPS2 with hypospermatogenesis and spermatogenic cell apoptosis.

Tue Aug 03 00:00:00 UTC 2021

生物信息学

蛋白别名: Phosphoribosyl pyrophosphate synthase II; PPRibP; PPRibP synthetase; PRS-II; ribose-phosphate diphosphokinase 2; Ribose-phosphate pyrophosphokinase 2; testicular secretory protein Li 41

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基因别名: PRPS2; PRSII

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UniProt ID: (Human) P11908

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Entrez Gene ID: (Human) 5634

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magnesium ion binding ribose phosphate diphosphokinase activity protein binding ATP binding kinase activity identical protein binding protein homodimerization activity nucleotide kinase

5-phosphoribose 1-diphosphate biosynthetic process nucleobase-containing compound metabolic process nucleoside metabolic process ribonucleoside monophosphate biosynthetic process nucleotide biosynthetic process phosphorylation