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FIGURE: 1 / 3
PRUNE2 Antibody (PA5-100310) in ICC/IF
Immunocytochemistry analysis of PRUNE2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PRUNE2 polyclonal antibody (Product # PA5-100310) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100310
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PRUNE2(Accession Q8WUY3), corresponding to amino acid residues V352-F402.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2849824
Product Specific Information
Antibody detects endogenous levels of total PRUNE2.
Target Information
PRUNE2 gene, also named BMCC1, encodes protein prune homolog 2. PRUNE2 gene are overlapping with PCA3, one of the most prostate cancer specific markers. PRUNE2 may play an important role in regulating differentiation, survival and aggressiveness of the tumor cells. Besides its high level of expression seen in the nervous system (brain, cerebellum and spinal cord), it is also expressed at high levels in noneuroblastoma, rhabdomyosarcoma, melanoma and some osteosarcoma cell lines, whereas at only low levels in cancer cell lines of liver, breast, thyroid and colon. The Calculated molecular weight of this protein is 340 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A214N16.3; bA214N16.3; BCH motif-containing molecule at the carboxyl terminal region 1; BNIP2 motif containing molecule at the carboxyl terminal region 1; BNIP2 motif-containing molecule at the C-terminal region 1; DKFZp762K117; neuronal protein; olfaxin; Protein prune homolog 2; RP11-214N16.3; RP11-58J3.2; truncated PRUNE2
Gene Aliases: 6330414G02Rik; A230083H22Rik; A330102H22Rik; BMCC1; BNIPXL; C9orf65; KIAA0367; mKIAA0367; PRUNE2; RGD1311350
UniProt ID: (Human) Q8WUY3, (Mouse) Q52KR3
Entrez Gene ID: (Human) 158471, (Mouse) 353211, (Rat) 293823
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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