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PRUNE2 Antibody (PA5-100310) in ICC/IF
Immunocytochemistry analysis of PRUNE2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PRUNE2 polyclonal antibody (Product # PA5-100310) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-100310
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PRUNE2(Accession Q8WUY3), corresponding to amino acid residues V352-F402.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2849824
产品详细信息
Antibody detects endogenous levels of total PRUNE2.
靶标信息
PRUNE2 gene, also named BMCC1, encodes protein prune homolog 2. PRUNE2 gene are overlapping with PCA3, one of the most prostate cancer specific markers. PRUNE2 may play an important role in regulating differentiation, survival and aggressiveness of the tumor cells. Besides its high level of expression seen in the nervous system (brain, cerebellum and spinal cord), it is also expressed at high levels in noneuroblastoma, rhabdomyosarcoma, melanoma and some osteosarcoma cell lines, whereas at only low levels in cancer cell lines of liver, breast, thyroid and colon. The Calculated molecular weight of this protein is 340 kDa.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: A214N16.3; bA214N16.3; BCH motif-containing molecule at the carboxyl terminal region 1; BNIP2 motif containing molecule at the carboxyl terminal region 1; BNIP2 motif-containing molecule at the C-terminal region 1; DKFZp762K117; neuronal protein; olfaxin; Protein prune homolog 2; RP11-214N16.3; RP11-58J3.2; truncated PRUNE2
基因别名: 6330414G02Rik; A230083H22Rik; A330102H22Rik; BMCC1; BNIPXL; C9orf65; KIAA0367; mKIAA0367; PRUNE2; RGD1311350
UniProt ID: (Human) Q8WUY3, (Mouse) Q52KR3
Entrez Gene ID: (Human) 158471, (Mouse) 353211, (Rat) 293823
