Invitrogen

PSD4 Polyclonal Antibody

PSD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PSD4 Antibody (PA5-31837) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PSD4 (Product # PA5-31837) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Product Image Gallery

PSD4 Antibody — Figure 3. KRAS* induces SDC1 membrane recycling through MAPK-PSD4-ARF6 axis. a-d , iKras p53 L/+ PDAC cells were grown in presence (ON) or absence (OFF) of doxycycline for 48 hours or indicated times. REON: cells were grown in absence of doxycycline for 48 hours followed by doxycycline treatment for 24 hours. Treatment of ON or REON samples with trametinib (50 nM), AZD8330 (100 nM), or BKM120 (100 nM) for 16-18 hours is indicated. ( a ) Surface Sdc1 was measured via FACS. ( b ) Cells were detached with trypsin to remove Sdc1 ectodomain and re-suspended in culture medium. (Top) Surface Sdc1 was labeled with anti-Sdc1 antibody at indicated times following re-suspension and measured via FACS. (Bottom) Mean Sdc1 levels. ( c ) Cells grown in presence (ON) or absence (OFF) of doxycycline for 48 hours were subjected to IF staining for Sdc1 (red), Arf6 (green) and DAPI (blue) (scale bar: 20 mum). ( d ) Cell lysates were immunoblotted for Psd4, phosphor-Akt, phosphor-Erk, Kras and ss-Actin. a-d: Data are representative of two independent experiments with similar results. {TM}

产品信息

PA5-31837

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 534 and 762 of Human PSD4

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2549310

产品详细信息

Recommended positive controls: A431.

Predicted reactivity: Mouse (89%), Rat (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Guanine nucleotide exchange factor for ARF6 and ARL14/ARF7. Through ARL14 activation, controls the movement of MHC class II-containing vesicles along the actin cytoskeleton in dendritic cells. Involved in membrane recycling. Interacts with several phosphatidylinositol phosphate species, including phosphatidylinositol 3,4-bisphosphate, phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 4,5-bisphosphate. [UniProt]

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Syndecan 1 is a critical mediator of macropinocytosis in pancreatic cancer.

Nature

Yao W,Rose JL,Wang W,Seth S,Jiang H,Taguchi A,Liu J,Yan L,Kapoor A,Hou P,Chen Z,Wang Q,Nezi L,Xu Z,Yao J,Hu B,Pettazzoni PF,Ho IL,Feng N,Ramamoorthy V,Jiang S,Deng P,Ma GJ,Den P,Tan Z,Zhang SX,Wang H,Wang YA,Deem AK,Fleming JB,Carugo A,Heffernan TP,Maitra A,Viale A,Ying H,Hanash S,DePinho RA,Draetta GF

PA5-31837 was used in Western Blotting to investigate if a mechanistic link between KRAS* signaling and a targetable molecule driving nutrient salvage pathway in pancreatic ductal adenocarcinoma exists.

Mon Apr 01 00:00:00 UTC 2019

生物信息学

蛋白别名: ADP-ribosylation factor guanine nucleotide-exchange factor 6; Exchange factor for ADP-ribosylation factor guanine nucleotide factor 6 B; exchange factor for ADP-ribosylation factor guanine nucleotide factor 6B; Exchange factor for ARF6 B; PH and SEC7 domain-containing protein 4; Pleckstrin homology and SEC7 domain-containing protein 4; SEC7 homolog; Telomeric of interleukin-1 cluster protein

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基因别名: EFA6B; PSD4; TIC

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UniProt ID: (Human) Q8NDX1

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Entrez Gene ID: (Human) 23550

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功能

ARF guanyl-nucleotide exchange factor activity phospholipid binding guanyl-nucleotide exchange factor

参与通路

regulation of ARF protein signal transduction positive regulation of GTPase activity