Invitrogen

PSEN2 Monoclonal Antibody (APS 21)

PSEN2 Antibody in Immunocytochemistry (ICC/IF)

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PSEN2 Antibody (MA1-753) in ICC/IF

Immunofluorescent analysis of Presenilin 2 using Anti-Presenilin 2 Monoclonal Antibody (APS 21) (Product # MA1-753) shows staining in NCI-H460 Cells. Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 (Product # MA1-753) at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated... View More

PSEN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PSEN2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of PSEN2 was observed in THP-1 when compared to Raji and Jurkat using Anti-PSEN2 Monoclonal Antibody (APS 21) (Product # MA1-753) in Western Blot. {RE}

产品信息

MA1-753

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

0.75 µg/mL

酶联免疫吸附实验 (ELISA)

4.5 µg/mL

其他PubMed (MISC)

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产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

APS 21

抗原

Synthetic peptide corresponding to residues C(31) Q E G R Q G P E D G E N T A(45) of human PS2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_325092

产品详细信息

MA1-753 detects presenilin 2 protein (PS2) from rat, mouse, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 1.

MA1-753 has successfully been used in immuno-fluorescence, Western blot and ELISA procedures. By Western blot, this antibody detects an ~28 kDa protein representing PS2 NT (N-terminus fragment) and the ~46 kDa full-length PS2 from transfected COS-7 cells. In 4% paraformaldehyde fixed COS-7 cells transfected with hPS2, MA1-753 showed specific Golgi, nuclear envelope, and ER labeling.

The MA1-753 immunogen is a synthetic peptide corresponding to residues C(31) Q E G R Q G P E D G E N T A(45) of human PS2.

靶标信息

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies.

Journal of neuroscience research

Diehlmann A,Ida N,Weggen S,Grünberg J,Haass C,Masters CL,Bayer TA,Beyreuther K

MA1-753 was used in ELISA, immunocytochemistry and western blot to study the presenilin 1 and presenilin 2 expression and processing by monoclonal antibodies