Invitrogen

PSMA5 Polyclonal Antibody

PSMA5 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

PSMA5 Antibody (PA1-1962) in ICC/IF

Immunofluorescence analysis of Proteasome 20S Subunit alpha-5 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSMA5 Rabbit Polyclonal (Product # PA1-1962) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj... View More

Product Image Gallery

产品信息

PA1-1962

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Hamster, Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues C A(120) L Q F G E E D A D P G A M(133) of human proteasome 20S subunit alpha 5.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2171717

产品详细信息

PA1-1962 detects proteasome 20S subunit alpha 5 from human, rat, mouse and hamster cell lysates.

PA1-1962 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a 26.5 kDa protein representing proteasome 20S subunit alpha 5 from human, rat, mouse, and hamster cell extracts.

PA1-1962 immunogen is a synthetic peptide corresponding to residues C A(120) L Q F G E E D A D P G A M(133) of human proteasome 20S subunit alpha 5. PA1-1962 immunizing peptide (Cat. # PEP-210) is available for use in neutralization and control experiments.

靶标信息

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics.

Cell reports

Markmiller S,Fulzele A,Higgins R,Leonard M,Yeo GW,Bennett EJ

PA1-1962 was used in Immunocytochemistry-immunoflourescence to study whether active ubiquitylation or neddylation modulates stress granule dynamics.

Tue Apr 30 00:00:00 UTC 2019

Network Analysis of UBE3A/E6AP-Associated Proteins Provides Connections to Several Distinct Cellular Processes.

Journal of molecular biology

Martínez-Noël G,Luck K,Kühnle S,Desbuleux A,Szajner P,Galligan JT,Rodriguez D,Zheng L,Boyland K,Leclere F,Zhong Q,Hill DE,Vidal M,Howley PM

Published figure using PSMA5 polyclonal antibody (Product # PA1-1962) in Western Blot

Fri Mar 30 00:00:00 UTC 2018

The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium.

The Journal of cell biology

Gerhardt C,Lier JM,Burmühl S,Struchtrup A,Deutschmann K,Vetter M,Leu T,Reeg S,Grune T,Rüther U

PA1-1962 was used in immunocytochemistry to use knockout mice to determine the function of RPGRIP1L.

Mon Jul 06 00:00:00 UTC 2015

ATM regulates proteasome-dependent subnuclear localization of TRF1, which is important for telomere maintenance.

Nucleic acids research

McKerlie M,Lin S,Zhu XD

PA1-1962 was used in western blot to study the role of ATM in telomere function

Tue May 01 00:00:00 UTC 2012

Urocortin trafficking in cerebral microvessel endothelial cells.

Journal of molecular neuroscience : MN

Tu H,Kastin AJ,Bjorbaek C,Pan W

PA1-1962 was used in immunocytochemistry to investigate the mechanism of urocortin endocytosis in cerebral microvessel endothelial cells

Mon Feb 04 00:00:00 UTC 2008

Neuronal induction of the immunoproteasome in Huntington's disease.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Díaz-Hernández M,Hernández F,Martín-Aparicio E,Gómez-Ramos P,Morán MA,Castaño JG,Ferrer I,Avila J,Lucas JJ

PA1-1962 was used in western blot to investigate the role of the ubiquitin-proteasome system during the neurodegeneration of huntington disease.

Wed Dec 17 00:00:00 UTC 2003

生物信息学

蛋白别名: epididymis tissue sperm binding protein Li 11n; macropain subunit zeta; Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; proteasome (prosome, macropain) subunit, alpha type, 5; proteasome alpha 5 subunit; proteasome component 5; Proteasome subunit alpha type-5; proteasome subunit alpha type-5-like protein; proteasome subunit zeta; Proteasome zeta chain

查看更多收起

基因别名: PSC5; PSMA5; ZETA

查看更多收起

UniProt ID: (Human) P28066, (Mouse) Q9Z2U1