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PSMC3 Antibody (MA5-49297) in ICC/IF
Immunocytochemistry analysis of PSMC3 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in PSMC3 Monoclonal antibody (Product # MA5-49297) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate fo... View More
Product Details
MA5-49297
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
4D3
Immunogen
E.coli-derived human TBP-1/PSMC3 recombinant protein (Position: M1-A439).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074955
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HEK293 whole cell, human Hela whole cell, human HepG2 whole cell, human PC-3 whole cell, rat stomach tissue, rat kidney tissue, mouse stomach tissue, mouse kidney tissue. IHC: human liver cancer tissue, human colonic adenocarcinoma tissue. ICC/IF: MCF-7 cell. Flow: U937 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a WASP interacting protein (WIP)-related protein. It has been shown that this protein has a role in the WASP-mediated organization of the actin cytoskeleton and that this protein is a potential link between the activated platelet-derived growth factor receptor and the actin polymerization machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 26S protease regulatory subunit 6A; 26S proteasome AAA-ATPase subunit RPT5; 26S proteasome regulatory subunit 6A; human immunodeficiency virus tat transactivator binding protein-1; MGC8487; proteasome (prosome, macropain) 26S subunit, ATPase, 3; Proteasome 26S subunit ATPase 3; Proteasome subunit P50; rpt5; Spermatogenic cell/sperm-associated Tat-binding protein homolog SATA; Tat binding protein 1; TAT-binding protein 1; TBP-1; testicular secretory protein Li 42
Gene Aliases: PSMC3; TBP-1; TBP1
UniProt ID: (Human) P17980, (Rat) Q63569, (Mouse) O88685
Entrez Gene ID: (Human) 5702, (Rat) 29677, (Mouse) 19182
MAPK cascade
protein polyubiquitination
blastocyst development
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
regulation of cellular amino acid metabolic process
viral process
ER-associated ubiquitin-dependent protein catabolic process
anaphase-promoting complex-dependent catabolic process
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of mRNA stability
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly
T cell receptor signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
Wnt signaling pathway, planar cell polarity pathway
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
positive regulation of proteasomal protein catabolic process
negative regulation of nucleic acid-templated transcription
protein catabolic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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