Invitrogen

PSME1 Polyclonal Antibody

图: 1 / 4

PSME1 Antibody (PA1-960) in WB

Western blot on PA28 alpha in Hepa 1 cell lysate using Product # PA1-960.

Product Image Gallery

PSME1 Antibody — IP-MS enrichment of PSME1 (LFQ intensity): PSME1 was enriched 22-fold from A549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and PSME1 antibody (Product # PA1-960). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

产品信息

PA1-960

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Dog, Hamster, Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: R(5) V Q P E A Q A K V D V F R E D L C(22)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2284415

产品详细信息

PA1-960 detects proteasome activator 11S REG alpha subunit from canine, hamster, human, mouse and rat tissues and cells.

PA1-960 has been successfully used in Western blot procedures. By Western blot, this antibody detects a 28 kDa protein representing 11S REG alpha subunit in rat brain extract.

PA1-960 immunizing peptide corresponds to amino acid residues 5-22 of human proteasome activator 11S REG alpha. This sequence differs from the mouse and rat 11S REG alpha protein by a single amino acid residue. PA1-960 immunizing peptide (Cat. # PEP-097) is available for use in neutralization and control experiments.

靶标信息

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (approximately 31), barrel-shaped molecular machine with an apparent molecular weight of approximately 2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Heterogeneous nuclear ribonucleoprotein A2 participates in the replication of Japanese encephalitis virus through an interaction with viral proteins and RNA.

Journal of virology

Katoh H,Mori Y,Kambara H,Abe T,Fukuhara T,Morita E,Moriishi K,Kamitani W,Matsuura Y

PA1-960 was used in western blot to investigate the interaction between heterogeneous nuclear ribonucleoprotein A2 and the Japanese encephalitis virus core protein and its involvement in viral replication

Tue Nov 01 00:00:00 UTC 2011

Characterization of the Brain 26S Proteasome and its Interacting Proteins.

Frontiers in molecular neuroscience

Tai HC,Besche H,Goldberg AL,Schuman EM

PA1-960 was used in western blot to detect the change in the proteasome-associated protein PA28alpha after NMDA exposure

Thu Jul 14 00:00:00 UTC 2011

Alterations of cerebral cortex and hippocampal proteasome subunit expression and function in a traumatic brain injury rat model.

Journal of neurochemistry

Yao X,Liu J,McCabe JT

PA1-960 was used in western blot to study the role of proteasome in a traumatic brain injury

Tue Jan 01 00:00:00 UTC 2008

Reduced ubiquitin C-terminal hydrolase-1 expression levels in dementia with Lewy bodies.

Neurobiology of disease

Barrachina M,Castaño E,Dalfó E,Maes T,Buesa C,Ferrer I

PA1-960 was used in western blot to investigate the changes of UCHL-1 in samples from dementia patients with Lewy bodies

Mon May 01 00:00:00 UTC 2006

Proteasome impairment does not contribute to pathogenesis in R6/2 Huntington's disease mice: exclusion of proteasome activator REGgamma as a therapeutic target.

Human molecular genetics

Bett JS,Goellner GM,Woodman B,Pratt G,Rechsteiner M,Bates GP

PA1-960 was used in western blot to investigate the role of the proteasome during the pathogenesis of R6/2 Huntington's disease.

Sun Jan 01 00:00:00 UTC 2006

生物信息学

蛋白别名: 11S regulator complex subunit alpha; 29-kD MCP activator subunit; Activator of multicatalytic protease subunit 1; epididymis secretory sperm binding protein Li 129m; IGUP I-5111; Interferon gamma up-regulated I-5111 protein; interferon-gamma IEF SSP 5111; interferon-gamma-inducible protein 5111; MGC8628; OTTHUMP00000164702; PA28 alpha subunit; PA28a; PA28alpha; protease (prosome, macropain) 28 subunit, alpha; proteasome (prosome, macropain) 28 subunit, alpha; proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); Proteasome activator 28 subunit alpha; Proteasome activator complex subunit 1; REG-alpha

查看更多收起

基因别名: AW413925; HEL-S-129m; I79_008549; IFI5111; PA28A; PA28alpha; PSME1; REGalpha

查看更多收起

UniProt ID: (Human) Q06323, (Mouse) P97371