Invitrogen

PSME3 Polyclonal Antibody

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PSME3 Antibody (38-3800) in WB

Western blot analysis was performed on whole cell extracts of Caco-2 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), Neuro-2a (Lane 4), PC-12 (Lane 5), COS-7 (Lane 6), Hep G2 (Lane 7), MOLT4 (Lane 8), Jurkat (Lane 9) and NIH/3T3 (Lane 10). The blots were probed with Anti-PA28-gamma Rabbit Polyclonal Antibody (Product # 38-3800, 2µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A27036, 0.4µg/mL, 1:2500 dilution). A ~ 31 kDa band corresponding to PA28-gamma wa... View More

PSME3 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

PSME3 Antibody — IP-MS enrichment of PSME3 (LFQ intensity): PSME3 was enriched 423-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and PSME3 antibody (Product # 38-3800). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

产品信息

38-3800

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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免疫细胞化学 (ICC/IF)

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免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human, Mouse, Non-human primate, Tag

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the N-terminal region of the human, mouse, rat and pig PA28-gamma (proteosome activator 28-gamma, Ki antigen) protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533368

靶标信息

SFRS10 contains 1 RRM (RNA recognition motif) domain and belongs to the splicing factor SR family. It is a sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

p53 is positively regulated by miR-542-3p.

Cancer research

Wang Y,Huang JW,Castella M,Huntsman DG,Taniguchi T

Sun Jun 15 00:00:00 UTC 2014

ARF regulates the stability of p16 protein via REGγ-dependent proteasome degradation.

Molecular cancer research : MCR

Kobayashi T,Wang J,Al-Ahmadie H,Abate-Shen C

Published figure using PSME3 polyclonal antibody (Product # 38-3800) in Western Blot

Thu Aug 01 00:00:00 UTC 2013

Regulation of REGγ cellular distribution and function by SUMO modification.

Cell research

Wu Y,Wang L,Zhou P,Wang G,Zeng Y,Wang Y,Liu J,Zhang B,Liu S,Luo H,Li X

38-3800 was used in immunocytochemistry and western blot to study the role of SUMOylation in the regulation of the REGgamma-proteasome pathway.

Sun May 01 00:00:00 UTC 2011

Regulation of REGγ cellular distribution and function by SUMO modification.

Cell research

Wu Y,Wang L,Zhou P,Wang G,Zeng Y,Wang Y,Liu J,Zhang B,Liu S,Luo H,Li X

38-3800 was used in immunocytochemistry and western blot to study the role of SUMOylation in the regulation of the REGgamma-proteasome pathway.

Sun May 01 00:00:00 UTC 2011

Regulation of REGγ cellular distribution and function by SUMO modification.

Cell research

Wu Y,Wang L,Zhou P,Wang G,Zeng Y,Wang Y,Liu J,Zhang B,Liu S,Luo H,Li X

38-3800 was used in immunocytochemistry and western blot to study the role of SUMOylation in the regulation of the REGgamma-proteasome pathway.

Sun May 01 00:00:00 UTC 2011

Ubiquitin- and ATP-independent proteolytic turnover of p21 by the REGgamma-proteasome pathway.

Molecular cell

Li X,Amazit L,Long W,Lonard DM,Monaco JJ,O'Malley BW

38-3800 was used in immunocytochemistry and western blot to report that p21 is a target of the REGgamma proteasome

Fri Jun 22 00:00:00 UTC 2007

Ubiquitin-independent degradation of cell-cycle inhibitors by the REGgamma proteasome.

Molecular cell

Chen X,Barton LF,Chi Y,Clurman BE,Roberts JM

38-3800 was used in western blot to explore how REGgamma contributes to p21 degradation

Fri Jun 22 00:00:00 UTC 2007

The SRC-3/AIB1 coactivator is degraded in a ubiquitin- and ATP-independent manner by the REGgamma proteasome.

Cell

Li X,Lonard DM,Jung SY,Malovannaya A,Feng Q,Qin J,Tsai SY,Tsai MJ,O'Malley BW

38-3800 was used in Immunoprecipitation to identify steroid receptor coactivator-3 as a physiologically relevant endogenous cellular target for the REGgamma-proteasome complex.

Fri Jan 27 00:00:00 UTC 2006

The SRC-3/AIB1 coactivator is degraded in a ubiquitin- and ATP-independent manner by the REGgamma proteasome.

Cell

Li X,Lonard DM,Jung SY,Malovannaya A,Feng Q,Qin J,Tsai SY,Tsai MJ,O'Malley BW

38-3800 was used in Immunoprecipitation to identify steroid receptor coactivator-3 as a physiologically relevant endogenous cellular target for the REGgamma-proteasome complex.

Fri Jan 27 00:00:00 UTC 2006

Aberrant accumulation of PTTG1 induced by a mutated thyroid hormone beta receptor inhibits mitotic progression.

The Journal of clinical investigation

Ying H,Furuya F,Zhao L,Araki O,West BL,Hanover JA,Willingham MC,Cheng SY

Wed Nov 01 00:00:00 UTC 2006

Aberrant accumulation of PTTG1 induced by a mutated thyroid hormone beta receptor inhibits mitotic progression.

The Journal of clinical investigation

Ying H,Furuya F,Zhao L,Araki O,West BL,Hanover JA,Willingham MC,Cheng SY

Wed Nov 01 00:00:00 UTC 2006

Aberrant accumulation of PTTG1 induced by a mutated thyroid hormone beta receptor inhibits mitotic progression.

The Journal of clinical investigation

Ying H,Furuya F,Zhao L,Araki O,West BL,Hanover JA,Willingham MC,Cheng SY

Wed Nov 01 00:00:00 UTC 2006

Aberrant accumulation of PTTG1 induced by a mutated thyroid hormone beta receptor inhibits mitotic progression.

The Journal of clinical investigation

Ying H,Furuya F,Zhao L,Araki O,West BL,Hanover JA,Willingham MC,Cheng SY

Wed Nov 01 00:00:00 UTC 2006

生物信息学

蛋白别名: 11S regulator complex gamma subunit; 11S regulator complex subunit gamma; Activator of multicatalytic protease subunit 3; epididymis secretory protein Li 283; Ki antigen; Ki nuclear autoantigen; PA28 gamma variant 5; PA28g; prosome macropain; proteaseome (prosome, macropain) 28 subunit, 3; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki); Proteasome activator 28 subunit gamma; proteasome activator 28-gamma; Proteasome activator complex subunit 3; REG gamma-3; REG-gamma

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基因别名: AA410043; Ab2-371; AU020960; HEL-S-283; Ki; PA28-gamma; PA28G; PA28gamma; PSME3; REG-GAMMA; REGgamma

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UniProt ID: (Human) P61289, (Mouse) Q13172

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Entrez Gene ID: (Human) 10197, (Mouse) 19192, (Rat) 287716

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功能

p53 binding protein binding identical protein binding endopeptidase activator activity MDM2/MDM4 family protein binding

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