Invitrogen

PSME3 Polyclonal Antibody

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PSME3 Antibody in Immunocytochemistry (ICC/IF)

PSME3 Antibody in Immunocytochemistry (ICC/IF)

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PSME3 Antibody (PA5-21789) in ICC/IF

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSME3 (Product # PA5-21789) antibody at 1:500 dilution.

PSME3 Antibody in Immunocytochemistry (ICC/IF)

PSME3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PSME3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PSME3 Antibody in Western Blot (WB)

PSME3 Antibody in Western Blot (WB)

PSME3 Antibody in Western Blot (WB)

PSME3 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

PSME3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

PSME3 Antibody in Immunohistochemistry (IHC)

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PSME3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSME3 (Product # PA5-21789) antibody at 1:500 dilution.

PSME3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of PSME3 was performed in paraffin-embedded mouse cervix tissue using PSME3 Polyclonal Antibody (Product # PA5-21789) at a dilution of 1:500.

PSME3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using PSME3 (Product # PA5-21789) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

PSME3 Antibody in Western Blot (WB) — Western Blot using PSME3 Polyclonal Antibody (Product # PA5-21789). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: H1299. Lane C: HCT116. Lane D: MCF-7. 12% SDS PAGE. PSME3 Polyclonal Antibody (Product # PA5-21789) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

PSME3 Antibody in Western Blot (WB) — Western Blot using PSME3 Polyclonal Antibody (Product # PA5-21789). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. Lane C: BCL-1. 12% SDS PAGE. PSME3 Polyclonal Antibody (Product # PA5-21789) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

PSME3 Antibody in Western Blot (WB) — Western Blot using PSME3 Polyclonal Antibody (Product # PA5-21789). Sample (30 µg of whole cell lysate). Lane A: PC-12. 12% SDS PAGE. PSME3 Polyclonal Antibody (Product # PA5-21789) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

PSME3 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP) — Fig. 5 BRAF can bind to PA28gamma and stabilize its protein level. A - C Western blot assays showed that the expression levels of BRAF were upregulated in a dosage-dependent and time-dependent manner. D , E A coimmunoprecipitation assay showed that exogenous Myc-BRAF and Flag-PA28gamma bind to each other in 293T cells. F , G Coimmunoprecipitation experiments showed that endogenous BRAF and PA28gamma interact with each other in epithelial cells. H After transfecting the Myc-BRAF plasmid into 293T cells, coimmunostaining showed that Myc-BRAF (red) colocalized with PA28gamma (green). Scale bar: 50 mum. I Immunoprecipitation assays showed that exogenous BRAF and MEK could simultaneously bind to PA28gamma. Immunoprecipitation of PA28gamma was performed with the Flag antibody. J Exogenous BRAF and PA28gamma were detected in immunoprecipitates performed with the HA antibody. K Exogenous MEK1 and PA28gamma can be detected in immunoprecipitates performed with the Myc antibody. L Transfection of 293T cells with the Myc-BRAF plasmid in a dose-dependent manner. Western blot assays showed that the expression levels of PA28gamma, p-MEK1, and p-ERK were significantly upregulated. M Immunoprecipitation shows that silencing BRAF expression reduces MEK1 binding to PA28gamma. N Western blot assays showed that knocking down PA28gamma inhibited BRAF-dependent expression of p-MEK1 and p-ERK.

PSME3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Arecoline can upregulate the expression levels of PA28gamma and phosphorylated MEK1 in epithelial cells, which induce EMT. Western blot assays showed that arecoline upregulated the expression levels of PA28gamma, p-MEK1, and p-ERK in an adosage-dependent (A) and time-dependent manner (B). C After 30 days of treatment of epithelial cells with low concentrations of arecoline (10 mug/mL), the morphology of epithelial cells transformed to a mesenchymal phenotype. Scale bar: 25 mum. Wound healing (D , E) and transwell assays F , G showed that the healing ability and migration ability of epithelial cells treated with arecoline were upregulated. Scale bar: 100 mum. Data represent the means +- s.d. of three independent experiments. Statistical analysis was performed using Student's t test (* p < 0.05, *** p < 0.001). H Representative results for immunostaining of PA28gamma, Vimentin, and E-cadherin in the normal control group and the arecoline-treated group. Scale bar: 50 mum. I Western blot assays showed the expression levels of Vimentin and E-cadherin in arecoline-treated epithelial cells.

PSME3 Antibody in Immunohistochemistry (IHC) — The expression of PA28gamma in OSF epithelial tissue was positively correlated with both MEK1 and phosphorylated MEK1. A Obtaining OSF malignant transformation genes through database search (GeneCards) and bioinformatics analysis. A total of 709 organ fibrosis genes were associated with pulmonary fibrosis (2349), liver fibrosis (1778), renal fibrosis (1792) and myocardial fibrosis (883). A Venn diagram yielded 7 genes associated with organ fibrosis (709), OSF (181) and OSCC (1693). Among them, 4 upregulated genes (KRT14, CTLA4, MEK1, and NR3C2) and 3 downregulated genes (CCL11, KRT8, and NYP) were obtained. B Correlation analysis showed that the MEK1 gene positively correlated with PA28gamma. C Masson's trichrome staining for identifying different stages of OSF phenotype, including early, mid, late, and OSCC with OSF. Scale bar: left panels, 500 mum; mid panels, 200 mum; right panels, 100 mum. D IHC staining and statistical analysis showed the expression of PA28gamma, MEK1, p-MEK1, and Ki67 in normal ( n = 10), OSF ( n = 52), and OSCC with OSF ( n = 18) tissues. Based on Student's t test (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.000). Scale bar: 200 mum. E - H Correlation analysis of 4 molecules in the normal, OSF and OSCC groups.

产品信息

PA5-21789

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

-

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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免疫沉淀 (IP)

-

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 254 of PSME3

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.65 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11155410

产品详细信息

Recommended positive controls: 293T, A549, H1299, HCT116, MCF-7, NIH-3T3, JC, BCL-1, PC-12.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (88%), Pig (100%), Chicken (94%), Chimpanzee (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

SFRS10 contains 1 RRM (RNA recognition motif) domain and belongs to the splicing factor SR family. It is a sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Identification of a BRAF/PA28γ/MEK1 signaling axis and its role in epithelial-mesenchymal transition in oral submucous fibrosis.

Cell death & disease

Xie C,Li Z,Hua Y,Sun S,Zhong L,Chen Q,Feng H,Ji N,Li T,Zhou X,Zeng X,Tang Z,Sun C,Li J,Chen Q

PA5-21789 was used in Immunohistochemistry to demonstrate that the expression of PA28γ was positively associated with MEK1 and gradually elevated from normal to progressive stages of OSF tissue.

Fri Aug 12 00:00:00 UTC 2022

Identification of a BRAF/PA28γ/MEK1 signaling axis and its role in epithelial-mesenchymal transition in oral submucous fibrosis.

Cell death & disease

Xie C,Li Z,Hua Y,Sun S,Zhong L,Chen Q,Feng H,Ji N,Li T,Zhou X,Zeng X,Tang Z,Sun C,Li J,Chen Q

PA5-21789 was used in Immunohistochemistry to demonstrate that the expression of PA28γ was positively associated with MEK1 and gradually elevated from normal to progressive stages of OSF tissue.

Fri Aug 12 00:00:00 UTC 2022

Identification of a BRAF/PA28γ/MEK1 signaling axis and its role in epithelial-mesenchymal transition in oral submucous fibrosis.

Cell death & disease

Xie C,Li Z,Hua Y,Sun S,Zhong L,Chen Q,Feng H,Ji N,Li T,Zhou X,Zeng X,Tang Z,Sun C,Li J,Chen Q

PA5-21789 was used in Immunohistochemistry to demonstrate that the expression of PA28γ was positively associated with MEK1 and gradually elevated from normal to progressive stages of OSF tissue.

Fri Aug 12 00:00:00 UTC 2022

Identification of a BRAF/PA28γ/MEK1 signaling axis and its role in epithelial-mesenchymal transition in oral submucous fibrosis.

Cell death & disease

Xie C,Li Z,Hua Y,Sun S,Zhong L,Chen Q,Feng H,Ji N,Li T,Zhou X,Zeng X,Tang Z,Sun C,Li J,Chen Q

PA5-21789 was used in Immunohistochemistry to demonstrate that the expression of PA28γ was positively associated with MEK1 and gradually elevated from normal to progressive stages of OSF tissue.

Fri Aug 12 00:00:00 UTC 2022

Identification of a BRAF/PA28γ/MEK1 signaling axis and its role in epithelial-mesenchymal transition in oral submucous fibrosis.

Cell death & disease

Xie C,Li Z,Hua Y,Sun S,Zhong L,Chen Q,Feng H,Ji N,Li T,Zhou X,Zeng X,Tang Z,Sun C,Li J,Chen Q

PA5-21789 was used in Immunohistochemistry to demonstrate that the expression of PA28γ was positively associated with MEK1 and gradually elevated from normal to progressive stages of OSF tissue.

Fri Aug 12 00:00:00 UTC 2022

An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity.

Nucleic acids research

Li D,Yang J,Malik V,Huang Y,Huang X,Zhou H,Wang J

PA5-21789 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that eIF4A3 post-transcriptionally controls the pluripotency-related cell cycle regulators and that its depletion causes the loss of pluripotency via cell cycle dysregulation.

Mon Nov 28 00:00:00 UTC 2022

生物信息学

蛋白别名: 11S regulator complex gamma subunit; 11S regulator complex subunit gamma; Activator of multicatalytic protease subunit 3; epididymis secretory protein Li 283; Ki antigen; Ki nuclear autoantigen; PA28 gamma variant 5; PA28g; prosome macropain; proteaseome (prosome, macropain) 28 subunit, 3; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki); Proteasome activator 28 subunit gamma; proteasome activator 28-gamma; Proteasome activator complex subunit 3; REG gamma-3; REG-gamma

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基因别名: AA410043; Ab2-371; AU020960; HEL-S-283; Ki; PA28-gamma; PA28G; PA28gamma; PSME3; REG-GAMMA; REGgamma

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UniProt ID: (Human) P61289, (Mouse) Q13172

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Entrez Gene ID: (Human) 10197, (Mouse) 19192, (Rat) 287716

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p53 binding protein binding identical protein binding endopeptidase activator activity MDM2/MDM4 family protein binding

MAPK cascade protein polyubiquitination stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent regulation of cellular amino acid metabolic process apoptotic process positive regulation of endopeptidase activity anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of proteasomal protein catabolic process negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of G1/S transition of mitotic cell cycle negative regulation of extrinsic apoptotic signaling pathway cell cycle