Invitrogen

PSME3 Recombinant Rabbit Monoclonal Antibody (20H9L19)

PSME3 Antibody in Immunocytochemistry (ICC/IF)

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PSME3 Antibody (700180) in ICC/IF

Immunofluorescent analysis of PSME3 in HeLa cells using a PSME3 recombinant rabbit monoclonal antibody (Product # 700180) at a dilution of 5 µg/mL in the absence of peptide (top) and presence of immunogenic peptide (bottom), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained with Alexa Fluor 568 Phalloidin (Product # A12380). Hoechst only (blue, left), AF488 signal only (green, middle) and composite image with Phalloidin (right).

PSME3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

PSME3 Antibody — IP-MS enrichment of PSME3 (LFQ intensity): PSME3 was enriched 753-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and PSME3 antibody (Product # 700180). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

产品信息

700180

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化（石蜡） (IHC (P))

2-4 µg/mL

免疫细胞化学 (ICC/IF)

4-6 µg/mL

免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

20H9L19

抗原

A peptide corresponding to amino acids 7-20 of P61289.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532292

产品详细信息

This antibody is predicted to react with canine, chicken, equine, orangutan, Xenopus and zebrafish based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

SFRS10 contains 1 RRM (RNA recognition motif) domain and belongs to the splicing factor SR family. It is a sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

PA28γ-20S proteasome is a proteolytic complex committed to degrade unfolded proteins.

Cellular and molecular life sciences : CMLS

Frayssinhes JA,Cerruti F,Laulin J,Cattaneo A,Bachi A,Apcher S,Coux O,Cascio P

700180 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to indicate that global stimulation of the degradation of unfolded proteins may represent a more general feature of PA28γ and suggest that this proteasomal activator might play a broader role in the pathway of protein degradation than previously believed.

Thu Dec 16 00:00:00 UTC 2021

Site-specific O-GlcNAcylation of Psme3 maintains mouse stem cell pluripotency by impairing P-body homeostasis.

Cell reports

Pecori F,Kondo N,Ogura C,Miura T,Kume M,Minamijima Y,Yamamoto K,Nishihara S

700180 was used in Immunoprecipitation to identify O-GlcNAcylation of proteasome activator subunit 3 protein as a node of the embryonic stem cell pluripotency network.

Tue Jul 13 00:00:00 UTC 2021

Mutant p53 (p53-R248Q) functions as an oncogene in promoting endometrial cancer by up-regulating REGγ.

Cancer letters

Wang H,Bao W,Jiang F,Che Q,Chen Z,Wang F,Tong H,Dai C,He X,Liao Y,Liu B,Sun J,Wan X

700180 was used in immunohistochemistry and western blot to demonstrate that the p53-R248Q mutant targets the proteasome activator REGgamma to promote endometrial cancer progression

Fri May 01 00:00:00 UTC 2015

生物信息学

蛋白别名: 11S regulator complex gamma subunit; 11S regulator complex subunit gamma; Activator of multicatalytic protease subunit 3; epididymis secretory protein Li 283; Ki antigen; Ki nuclear autoantigen; PA28 gamma variant 5; PA28g; prosome macropain; proteaseome (prosome, macropain) 28 subunit, 3; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki); Proteasome activator 28 subunit gamma; proteasome activator 28-gamma; Proteasome activator complex subunit 3; REG gamma-3; REG-gamma

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基因别名: AA410043; Ab2-371; AU020960; HEL-S-283; Ki; PA28-gamma; PA28G; PA28gamma; PSME3; REG-GAMMA; REGgamma

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UniProt ID: (Human) P61289, (Mouse) Q13172

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Entrez Gene ID: (Human) 10197, (Mouse) 19192, (Rat) 287716

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功能

p53 binding protein binding identical protein binding endopeptidase activator activity MDM2/MDM4 family protein binding

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