Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 3
PTGER3 Antibody (PA5-102057) in ICC/IF
Immunocytochemistry analysis of PTGER3 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PTGER3 polyclonal antibody (Product # PA5-102057) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-102057
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PTGER3(Accession P43115), corresponding to amino acid residues L353-R390.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2851487
产品详细信息
Antibody detects endogenous levels of total PTGER3.
靶标信息
CD172a, also known as signal-regulatory protein alpha, is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: Ep3 receptor; MGC141828; MGC141829; MGC27302; PGE receptor EP3 subtype; PGE receptor, EP3 subtype; PGE2 receptor EP3 subtype; PGE2-R; pr; prostaglandin E receotor EP3 subtype 3 isoform; prostaglandin E receptor 3 (subtype EP3); Prostaglandin E2 receptor EP3 subtype; prostaglandin receptor (PGE-2); Prostanoid EP3 receptor
基因别名: EP3; EP3-I; EP3-II; EP3-III; EP3-IV; EP3-VI; EP3e; PGE2-R; PTGER3
UniProt ID: (Human) P43115
Entrez Gene ID: (Human) 5733
inflammatory response
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
female pregnancy
cell death
positive regulation of gene expression
negative regulation of norepinephrine secretion
intestine smooth muscle contraction
urinary bladder smooth muscle contraction
negative regulation of cAMP biosynthetic process
positive regulation of cAMP biosynthetic process
positive regulation of fever generation
response to estradiol
response to progesterone
response to drug
positive regulation of vasoconstriction
positive regulation of circadian sleep/wake cycle, non-REM sleep
negative regulation of insulin secretion
maternal process involved in parturition
negative regulation of gastric acid secretion
cellular response to lipopolysaccharide
cellular response to interleukin-1
negative regulation of platelet aggregation
negative regulation of potassium ion transmembrane transport
response to salt
negative regulation of calcium ion transmembrane transport
negative regulation of gastrin-induced gastric acid secretion
positive regulation of smooth muscle contraction involved in micturition
cellular response to forskolin
positive regulation of inhibitory G-protein coupled receptor phosphorylation
negative regulation of circadian sleep/wake cycle, wakefulness
positive regulation of myofibroblast contraction
positive regulation of colon smooth muscle contraction
positive regulation of gastric mucosal blood circulation
prostaglandin receptor internalization
positive regulation of neutrophil extravasation
negative regulation of forebrain neuron differentiation
