Invitrogen

PTGIR Polyclonal Antibody

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抗体检测数据 (2)

PTGIR Antibody in Immunocytochemistry (ICC/IF)

PTGIR Antibody in Immunocytochemistry (ICC/IF)

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PTGIR Antibody (PA5-102240) in ICC/IF

Immunocytochemistry analysis of PTGIR in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PTGIR polyclonal antibody (Product # PA5-102240) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

PTGIR Antibody in Immunocytochemistry (ICC/IF)

PTGIR Antibody in Western Blot (WB)

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PTGIR Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of PTGIR in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PTGIR polyclonal antibody (Product # PA5-102240) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

PTGIR Antibody in Western Blot (WB) — Western blot analysis of PTGIR in K562 cells. Samples were incubated with PTGIR polyclonal antibody (Product # PA5-102240).

产品信息

PA5-102240

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human PTGIR(Accession P43119), corresponding to amino acid residues G204-P254.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2851657

产品详细信息

Antibody detects endogenous levels of total Prostacyclin Receptor.

靶标信息

Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: IP receptor; PGI receptor; PGI2 receptor; Prostacyclin receptor; Prostaglandin I2 receptor; Prostanoid IP receptor

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基因别名: IP; PRIPR; PTGIR

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UniProt ID: (Human) P43119

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Entrez Gene ID: (Human) 5739

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guanyl-nucleotide exchange factor activity prostacyclin receptor activity G-protein coupled receptor

inflammatory response G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger adenylate cyclase-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration cell-cell signaling negative regulation of platelet-derived growth factor receptor signaling pathway positive regulation of cAMP biosynthetic process response to lipopolysaccharide positive regulation of GTPase activity positive regulation of cAMP-mediated signaling negative regulation of smooth muscle cell proliferation cellular response to prostaglandin D stimulus