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PTH Antibody (MA5-42935) in WB
Western blot analysis of PTH in extracts of various cell lines. Samples were incubated with PTH Monoclonal antibody (Product # MA5-42935) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-42935
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5X6R6
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Parathyroid Hormone (PTH) (P01270).
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2912076
Product Specific Information
Positive test controls include: Mouse thymus, Rat thymus, Jurkat. The target is usually found in the following locations: Secreted.
Immunogen sequence: MIPAKDMAKV MIVMLAICFL TKSDGKSVKK RSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE
Target Information
This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hypothalamic parathyroid hormone; Parathyrin; Parathyroid hormone; PTH
Gene Aliases: Pth; PTH-(1-84); Pth1; Pthp; Pthr1
UniProt ID: (Rat) P04089
Entrez Gene ID: (Rat) 24694, (Mouse) 19226
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
ossification
transcription from RNA polymerase II promoter
cellular calcium ion homeostasis
adenylate cyclase-activating G-protein coupled receptor signaling pathway
Rho protein signal transduction
cell-cell signaling
positive regulation of signal transduction
response to lead ion
regulation of gene expression
positive regulation of bone mineralization
positive regulation of cAMP biosynthetic process
response to nutrient levels
response to vitamin D
cellular macromolecule biosynthetic process
response to drug
bone resorption
response to ethanol
positive regulation of glycogen biosynthetic process
positive regulation of ossification
positive regulation of transcription from RNA polymerase II promoter
positive regulation of glucose import
response to cadmium ion
response to parathyroid hormone
response to fibroblast growth factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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