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PTP1B Antibody (MA5-32488) in ICC/IF
Immunofluorescence analysis of PTPN1 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with PTPN1 Monoclonal Antibody (Product # MA5-32488) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody (Product # A28177) at a dilution of ... View More
产品信息
MA5-32488
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Zebrafish
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JJ0935
抗原
Synthetic peptide within Human PTP1B aa 374-400
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809765
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
PTP1B (PTPN1) is a tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. It mediates dephosphorylation of EIF2AK3/PERK and in turn inactivates the protein kinase activity of EIF2AK3/PERK. PTP1B also plays a role in CKII- and p60c-src-induced signal transduction cascades. It regulates the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. PTP1B also regulates the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CPTP1; EC 3.1.3.48; hgnc9642; KIAA0283; protein tyrosine phosphatase 1b; protein tyrosine phosphatase, placental; Protein-tyrosine phosphatase 1B; PTN1; PTP-1B; RP5-1121H13.2; RPTPrho; tyrosine phosphatase 1b; tyrosine-phosphatase; Tyrosine-protein phosphatase non-receptor type 1
基因别名: PTP1B; PTPN1; wu:fk54h03
UniProt ID: (Human) P18031
Entrez Gene ID: (Human) 5770, (Zebrafish) 30081
protein tyrosine phosphatase activity
insulin receptor binding
protein binding
zinc ion binding
enzyme binding
protein kinase binding
receptor tyrosine kinase binding
poly(A) RNA binding
ephrin receptor binding
protein phosphatase 2A binding
cadherin binding involved in cell-cell adhesion
phosphoprotein phosphatase activity
hydrolase activity
phosphatase activity
protein phosphatase
protein dephosphorylation
activation of signaling protein activity involved in unfolded protein response
activation of JUN kinase activity
insulin receptor signaling pathway
regulation of signal transduction
negative regulation of signal transduction
regulation of endocytosis
negative regulation of vascular endothelial growth factor receptor signaling pathway
endoplasmic reticulum unfolded protein response
actin cytoskeleton reorganization
regulation of intracellular protein transport
peptidyl-tyrosine dephosphorylation
platelet-derived growth factor receptor-beta signaling pathway
IRE1-mediated unfolded protein response
negative regulation of MAP kinase activity
negative regulation of insulin receptor signaling pathway
regulation of type I interferon-mediated signaling pathway
JAK-STAT cascade involved in growth hormone signaling pathway
positive regulation of protein tyrosine kinase activity
negative regulation of ERK1 and ERK2 cascade
cell-cell adhesion
regulation of hepatocyte growth factor receptor signaling pathway
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of IRE1-mediated unfolded protein response
negative regulation of PERK-mediated unfolded protein response
peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity
positive regulation of receptor catabolic process
dephosphorylation
