Invitrogen

PU.1 Monoclonal Antibody (E.388.3)

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PU.1 Antibody (MA5-15064) in ChIP

Chromatin Immunoprecipitation (ChIP) assay of endogenous PU.1 protein using Anti-PU.1 Antibody: ChIP was performed using Anti-PU.1 Rabbit Monoclonal Antibody (Product # MA5-15064, 5 µg) on sheared chromatin from THP1 cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to SLC35C1 and PTPN6 promoter and SATA satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG usin... View More

PU.1 Antibody in Immunocytochemistry (ICC/IF)

PU.1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PU.1 Antibody in Western Blot, ChIP Assay (WB, ChIP)

PU.1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

PU.1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of PU.1 was observed in K-562, Daudi, RAW 264.7 in comparison to HeLa and 3T3-L1 using Anti-PU.1 Monoclonal Antibody (E.388.3) (Product # MA5-15064) in Western Blot. {RE}

产品信息

MA5-15064

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:800

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:150-1:200

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

染色质免疫沉淀实验 (ChIP)

2.5 µg/10^6 cells

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

E.388.3

抗原

Synthetic peptide corresponding to the sequence of human PU.1 protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

8 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_10986949

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

KDM6A Lysine Demethylase Directs Epigenetic Polarity of MDSCs during Murine Sepsis.

Journal of innate immunity

Bah I,Alkhateeb T,Youssef D,Yao ZQ,McCall CE,El Gazzar M

Published figure using PU.1 monoclonal antibody (Product # MA5-15064) in ChIP assay

Wed Apr 06 00:00:00 UTC 2022

KDM6A Lysine Demethylase Directs Epigenetic Polarity of MDSCs during Murine Sepsis.

Journal of innate immunity

Bah I,Alkhateeb T,Youssef D,Yao ZQ,McCall CE,El Gazzar M

Published figure using PU.1 monoclonal antibody (Product # MA5-15064) in ChIP assay

Wed Apr 06 00:00:00 UTC 2022

Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis.

Frontiers in immunology

Bah I,Youssef D,Yao ZQ,McCall CE,El Gazzar M

MA5-15064 was used in ChIP Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that epigenetic targeting of MDSCs in human sepsis might resolve post-sepsis immunosuppression and improve sepsis survival.

Mon Mar 28 00:00:00 UTC 2022

Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis.

Frontiers in immunology

Bah I,Youssef D,Yao ZQ,McCall CE,El Gazzar M

Mon Mar 28 00:00:00 UTC 2022

Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis.

Frontiers in immunology

Bah I,Youssef D,Yao ZQ,McCall CE,El Gazzar M

Mon Mar 28 00:00:00 UTC 2022

Genomic and epigenomic adaptation in SP-R210 (Myo18A) isoform-deficient macrophages.

Immunobiology

Yau E,Chen Y,Song C,Webb J,Carillo M,Kawasawa YI,Tang Z,Takahashi Y,Umstead TM,Dovat S,Chroneos ZC

MA5-15064 was used in ChIP assay to suggest that the inflammatory differences seen in SP-R210L-deficient macrophages are a result of transcriptional differences that are mediated by epigenetic changes brought about by differential expression of the SP-R210 isoforms.

Mon Nov 01 00:00:00 UTC 2021

Genomic and epigenomic adaptation in SP-R210 (Myo18A) isoform-deficient macrophages.

Immunobiology

Yau E,Chen Y,Song C,Webb J,Carillo M,Kawasawa YI,Tang Z,Takahashi Y,Umstead TM,Dovat S,Chroneos ZC

Mon Nov 01 00:00:00 UTC 2021

TH9 cells that express the transcription factor PU.1 drive T cell-mediated colitis via IL-9 receptor signaling in intestinal epithelial cells.

Nature immunology

Gerlach K,Hwang Y,Nikolaev A,Atreya R,Dornhoff H,Steiner S,Lehr HA,Wirtz S,Vieth M,Waisman A,Rosenbauer F,McKenzie AN,Weigmann B,Neurath MF

MA5-15064 was used in immunohistochemistry - frozen section to study the role of TH9 cells in ulcerative colitis

Tue Jul 01 00:00:00 UTC 2014

生物信息学

蛋白别名: 31 kDa transforming protein; 31 kDa-transforming protein; 31kDa Transforming Protein; hCG_25181; hematopoietic transcription factor PU.1; SFFV proviral integration 1 protein; SPIA; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1; Transcription Factor spi1

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基因别名: Dis-1; Dis1; OF; PU.1; Sfpi-1; SFPI1; SPI-1; SPI-A; SPI1; Tcfpu1; Tfpu.1

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UniProt ID: (Human) P17947, (Mouse) P17433

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Entrez Gene ID: (Human) 6688, (Mouse) 20375

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding RNA binding protein binding NFAT protein binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding sequence-specific DNA binding winged helix/forkhead transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter lymphoid progenitor cell differentiation transcription from RNA polymerase II promoter lymphocyte differentiation erythrocyte differentiation macrophage differentiation granulocyte differentiation somatic stem cell population maintenance myeloid dendritic cell differentiation histone H3 acetylation hypermethylation of CpG island negative regulation of MHC class II biosynthetic process regulation of erythrocyte differentiation negative regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter anatomical structure regression cellular response to ethanol negative regulation of histone H4 acetylation apoptotic process involved in patterning of blood vessels positive regulation of pri-miRNA transcription from RNA polymerase II promoter vasculature development myeloid leukocyte differentiation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter

PU.1 Monoclonal Antibody (E.388.3)

PU.1 Antibody (MA5-15064) in ChIP

产品信息

MA5-15064

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

PU.1 Monoclonal Antibody (E.388.3)

PU.1 Antibody (MA5-15064) in ChIP

产品信息

MA5-15064

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)