Invitrogen

PYGO2 Monoclonal Antibody (539730)

PYGO2 Antibody in Immunocytochemistry (ICC/IF)

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PYGO2 Antibody (MA5-24240) in ICC/IF

Immunocytochemistry analysis of PYGO2 in immersion fixed MCF‚7 human breast cancer cell line. Samples were incubated in PYGO2 monoclonal antibody (Product # MA5-24240) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.

PYGO2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-24240

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-5 µg/mL

免疫组化（石蜡） (IHC (P))

3-25 µg/mL

免疫细胞化学 (ICC/IF)

8-25 µg/mL

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

539730

抗原

E. coli-derived recombinant human Pygopus-2 Met160-Glu336

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2605750

产品详细信息

In direct ELISAs, no cross-reactivity with recombinant human Pygopus-1 or recombinant mouse Pygopus-1 is observed.

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

靶标信息

Involved in signal transduction through the Wnt pathway. [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Discovery of an Orally Bioavailable Small-Molecule Inhibitor for the β-Catenin/B-Cell Lymphoma 9 Protein-Protein Interaction.

Journal of medicinal chemistry

Wang Z,Zhang M,Quereda V,Frydman SM,Ming Q,Luca VC,Duckett DR,Ji H

MA5-24240 was used in Chromatin immunoprecipitation to report a drug-like small molecule, ZW4864, that shows good pharmacokinetic properties and selectively disrupts the protein-protein interaction between B-cell lymphoma 9 (BCL9) and β-catenin.