Invitrogen

PYROXD1 Polyclonal Antibody

查看其他3个PYROXD1抗体

抗体检测数据 (6)

PYROXD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PYROXD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PYROXD1 Antibody (PA5-114404) in IHC (P)

Immunohistochemistry analysis of PYROXD1 in paraffin-embedded human mammary cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PYROXD1 polyclonal antibody (Product # PA5-114404) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) ... View More

PYROXD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PYROXD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PYROXD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PYROXD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PYROXD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PYROXD1 Antibody in Western Blot (WB)

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PYROXD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PYROXD1 in paraffin-embedded human mammary cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PYROXD1 polyclonal antibody (Product # PA5-114404) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

PYROXD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PYROXD1 in paraffin-embedded human colon cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PYROXD1 polyclonal antibody (Product # PA5-114404) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

PYROXD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PYROXD1 in paraffin-embedded human lung cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PYROXD1 polyclonal antibody (Product # PA5-114404) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

PYROXD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PYROXD1 in paraffin-embedded mouse intestine tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PYROXD1 polyclonal antibody (Product # PA5-114404) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

PYROXD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PYROXD1 in paraffin-embedded rat intestine tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PYROXD1 polyclonal antibody (Product # PA5-114404) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

PYROXD1 Antibody in Western Blot (WB) — Western blot analysis of PYROXD1 in the following samples: Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: rat kidney tissue lysates. Samples consisting of 50 µg (reducing conditions) of protein was separated with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V, Resolving gel; 2-3 hrs.), transferred to a Nitrocellulose membrane (150mA, 50-90 min) and washed with TBS-0.1% Tween (3 times, 5 minutes/wash) and blocked with 5% Non-fat Milk/TBS (1.5 hrs., room temperature). The membrane was incubated in PYROXD1 polyclonal antibody (Product # PA5-114404) at a dilution of 0.5 µg/mL (overnight, 4°C), followed by goat anti-rabbit IgG-HRP and chemiluminescence (ECL) with a dilution of 1:10,000 (1.5 hours, room temperature).

产品信息

PA5-114404

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

-

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

-

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human PYROXD1 recombinant protein (Position: K48-N230).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2884860

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FLJ22028; Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1

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基因别名: PYROXD1

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UniProt ID: (Human) Q8WU10, (Rat) Q68FS6, (Mouse) Q3TMV7

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Entrez Gene ID: (Human) 79912, (Rat) 297708, (Mouse) 232491

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protein binding oxidoreductase activity molecular_function alkyl hydroperoxide reductase activity oxidoreductase

oxidation-reduction process