Invitrogen

Paxillin Polyclonal Antibody

Paxillin Antibody in Immunocytochemistry (ICC/IF)

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Paxillin Antibody (PA5-79899) in ICC/IF

Immunocytochemistry analysis of PXN using anti-PXN antibody (Product # PA5-79899) . PXN was detected in a section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-PXN antibody (Product # PA5-79899) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize... View More

Paxillin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Paxillin Antibody in Immunohistochemistry (Frozen) (IHC (F))

Paxillin Antibody in Flow Cytometry (Flow)

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产品信息

PA5-79899

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

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免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminal of human Paxillin (456-472aa HEKDGKAYCRKDYFDMF).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide, 0.05mg thimerosal

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2747014

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Vaccine against gastrin, a polyclonal antibody stimulator, decreases pancreatic cancer metastases.

American journal of physiology. Gastrointestinal and liver physiology

Osborne N,Sundseth R,Gay MD,Cao H,Tucker RD,Nadella S,Wang S,Liu X,Kroemer A,Sutton L,Cato A,Smith JP

PA5-79899 was used in Western Blotting to investigate the effects of lone and combined treatment of polyclonal antibody stimulator, an anti-gastrin cancer vaccine, on pancreatic cancer growth, metastases, and the tumor microenvironment.

Fri Nov 01 00:00:00 UTC 2019

生物信息学

蛋白别名: FLJ16691; hCG_1778014; myocardial ischemic preconditioning associated protein 7; PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134

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基因别名: PXN

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UniProt ID: (Human) P49023

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Entrez Gene ID: (Human) 5829, (Rat) 360820

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功能

integrin binding protein binding beta-catenin binding zinc ion binding vinculin binding protein kinase binding protein complex binding BH4 domain binding actin or actin-binding cytoskeletal protein

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