Invitrogen

Perforin Monoclonal Antibody (dG9 (delta G9)), eBioscience™

Perforin Antibody in Immunohistochemistry (IHC)

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Perforin Antibody (14-9994-82) in IHC

Science translational medicine 2013 - Published figure using Perforin monoclonal antibody (Product # 14-9994-82) in Immunohistochemistry

Perforin Antibody in Flow Cytometry (Flow)

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产品信息

14-9994-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

Assay-Dependent

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

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免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Human, Pig

已发表种属

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

dG9 (delta G9)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_468677

产品详细信息

Description: The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.

Applications Reported: This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. (Fluorochrome conjugated dG9 (delta G9) is recommended for use in intracellular flow cytometry.).

Applications Tested: This dG9 (delta G9) antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Thu Mar 03 00:00:00 UTC 2022

Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma.

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Liu X,Jin S,Hu S,Li R,Pan H,Liu Y,Lai P,Xu D,Sun J,Liu Z,Gao Y,Zhao Y,Liu F,Xiao Y,Li Y,Wen Y,Chen Z,Xu B,Lin Y,Ran M,Li Q,Yang S,Li H,Tu P,Haniffa M,Teichmann SA,Bai F,Wang Y

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Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

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14-9994 was used in Flow cytometry/Cell sorting to analyse the phenotype and function of Vδ2 T cells in HCV-infected patients compared to healthy controls.

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Lipoxin A4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma.

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生物信息学

蛋白别名: Cytolysin; lymphocyte pore forming protein; Lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1 (pore forming protein); Perforin-1; Perforin1; PFP; PGFL; PIGF; PIGF-2; PLGF; pore-forming protein; PRF1 (pore forming protein 1); RP11-710A11.3

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基因别名: FLH2; HPLH2; P1; PFN1; PFP; PRF1

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UniProt ID: (Human) P14222

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Entrez Gene ID: (Human) 5551, (Pig) 396595

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功能

calcium ion binding protein binding wide pore channel activity

参与通路

immunological synapse formation defense response to tumor cell immune response to tumor cell apoptotic process cellular defense response cytolysis protein homooligomerization defense response to virus transmembrane transport