Invitrogen

Perilipin 1 Polyclonal Antibody, Alexa Fluor™ 555

Perilipin 1 Antibody in Immunocytochemistry (ICC/IF)

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Perilipin 1 Antibody (PA1-1051-A555) in ICC/IF

Immunofluorescent analysis of Perilipin (red) in HepG2 cells. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were stained with a Perilipin rabbit polyclonal antibody (Product # PA1-1051-A555) at a concentration of 5 µg/mL in blocking buffer for 1 hour at room temperature. Nuc... View More

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产品信息

PA1-1051-A555

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the residues C E(502) P I L G R T Q Y S Q L R K K S(517) of rat Perilipin A.

查看免疫原

偶联物

Alexa Fluor™ 555 Alexa Fluor™ 555 Alexa Fluor™ 555

激发/发射光谱

553/568 nm 查看光谱

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1.0mg/mL BSA

内含物

0.05% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2723454

产品详细信息

PA1-1051 detects perilipin A from rat and 3T3-L1 cell extract. The PA1-1051 immunogen is a synthetic peptide corresponding to the residues C E(502) P I L G R T Q Y S Q L R K K S(517) of rat Perilipin A. This sequence is 93% conserved in humans. The PA1-1051 immunizing peptide (Cat. # PEP-169) is available for use in neutralization and control experiments.

靶标信息

Adipose tissue is an energy reserve in animals and is strictly regulated in nondomestic species. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), resistin, and perilipin. Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.

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