Invitrogen

Periplakin Polyclonal Antibody

Periplakin Antibody in Immunocytochemistry (ICC/IF)

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Periplakin Antibody (PA5-79856) in ICC/IF

Immunocytochemical analysis of Periplakin in A431 cells using anti-Periplakin antibody (Product # PA5-79856). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-Periplakin Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1... View More

Periplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Periplakin Antibody in Western Blot (WB)

Periplakin Antibody in Flow Cytometry (Flow)

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产品信息

PA5-79856

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.25 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human Periplakin (1664-1701aa DTGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVK).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2746971

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.

仅用于科研。不用于诊断过程。未经明确授权不得转售。