Invitrogen

Phospho-A-Raf/B-Raf/C-Raf (Ser471, Ser579, Ser432) Polyclonal Antibody

Phospho-A-Raf/B-Raf/C-Raf (Ser471, Ser579, Ser432) Antibody in Western Blot (WB)

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Phospho-A-Raf/B-Raf/C-Raf (Ser471, Ser579, Ser432) Antibody (PA5-143757) in WB

Western blot of Phospho-A-Raf/B-Raf/C-Raf (Ser471, Ser579, Ser432) in human Jurkat cells treated with calyculin A (100 nM) for 30 minutes. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-C-Raf (N-terminal region) (lanes 1 & 2) or Phospho-A-Raf/B-Raf/C-Raf (Ser471, Ser579, Ser432) polyclonal antibody (Product # PA5-143757) (lanes 3 & 4).

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产品信息

PA5-143757

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-471 in human C-Raf. This sequence has high homology with similar regions in rat and mouse C-Raf, and has high homology to the conserved sites in B-Raf (Ser-579) and A-Raf (Ser-432).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA, 50% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2942985

产品详细信息

This antibody was cross-adsorbed to phospho-C-Raf (Ser-338) peptide before affinity purification using phospho-C-Raf (Ser-471) peptide. The purified antibody detects a band at 74 kDa* corresponding to C-Raf in western blots of human Jurkat cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.

靶标信息

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (Ser259)/B-Raf(Ser365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain. Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation. Ser-579 is required for growth factor activation and kinase activity. Thus, multiple sites of phosphorylation within Rafs may be important for regulation of their activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF1; C-RAF; C-Raf proto-oncogene, serine/threonine kinase; CRaf; Craf1 transforming; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); murine sarcoma 3611 oncogene 1; murine sarcoma viral (v-raf) oncogene homolog B1; Oncogene RAF1; p94; Proto-oncogene B-Raf; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; Raf1; RAFB1; sarcoma 3611 oncogene; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1; v-raf-1 leukemia viral oncogene 1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1

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基因别名: 6430402F14Rik; 9930012E13Rik; AA120551; AA387315; AA473386; AA990557; B-raf; B-RAF1; BB129353; BRAF; Braf-2; BRAF1; Braf2; c-Raf; C230098H17; C87398; CMD1NN; CRAF; Craf1; D6Ertd631e; D830050J10Rik; NS5; NS7; RAF; Raf-1; RAF1; RAFB1; v-Raf