Invitrogen

Phospho-AMOT (Ser175) Recombinant Rabbit Monoclonal Antibody (18H4L17)

Phospho-AMOT (Ser175) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-AMOT (Ser175) Antibody (702980) in ICC/IF

For immunofluorescence analysis, HEK-293 cells were fixed and permeabilized for detection of endogenous AMOT pS175 using Anti-Phopho-AMOT (Ser175) Recombinant Rabbit Monoclonal Antibody (Product # 702980, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond... View More

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产品信息

702980

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

18H4L17

抗原

Peptide corresponding to human AMOT[pS175] [aa172-aa179]

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2762384

产品详细信息

This antibody is predicted to react with Monkey, Sheep, Rabbit, Mouse.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

AMOT (Angiomotin) belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. AMOT plays a central role in tight junction maintenance via the complex formed with ARHGAP17 - which acts by regulating the uptake of polarity proteins at tight junctions. AMOT is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels.

仅用于科研。不用于诊断过程。未经明确授权不得转售。