Invitrogen

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody (44-1150G) in ICC/IF

Immunofluorescence analysis of Phospho-AMPK alpha 1,2 (Thr183, Thr172) was done on 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-AMPK alpha-1,2 (Thr183, Thr172) Rabbit Polyclonal Antibody (Product # 44-115... View More

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody

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产品信息

44-1150G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:50

免疫细胞化学 (ICC/IF)

1:250

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human AMPK alpha 1 that contains threonine 183 and AMPK alpha 2 that contains threonine 172. The sequence is conserved in human, mouse and rat.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533585

靶标信息

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

AMPK hyperactivity maintains the survival of vasculogenic T cells in patients with Takayasu's arteritis.

Clinical and experimental rheumatology

Sun M,Zhang X,Hao Y,Yin P,Hu J,Zheng M,Wen Z,Liu X,Wang C

44-1150G was used in Flow Cytometry to investigate the mechanisms underlying the maintenance of vasculogenic proinflammatory T cells in Takayasu's arteritis (TAK) patients.

Sat Apr 01 00:00:00 UTC 2023

Glycocalyx heparan sulfate cleavage promotes endothelial cell angiopoietin-2 expression by impairing shear stress-related AMPK/FoxO1 signaling.

JCI insight

Richter RP,Ashtekar AR,Zheng L,Pretorius D,Kaushlendra T,Sanderson RD,Gaggar A,Richter JR

44-1150G was used in Immunocytochemistry-immunoflourescence to establish a paradigm by which Ang-2 may be upregulated during sepsis.

Mon Aug 08 00:00:00 UTC 2022

OR2AT4, an Ectopic Olfactory Receptor, Suppresses Oxidative Stress-Induced Senescence in Human Keratinocytes.

Antioxidants (Basel, Switzerland)

Kim JS,Lee HL,Jeong JH,Yoon YE,Lee IR,Kim JM,Wu C,Lee SJ

44-1150G was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest that OR2AT4 may be a novel therapeutic target for anti-aging and anti-senescence in human keratinocytes.

Thu Nov 03 00:00:00 UTC 2022

ACSL4 contributes to sevoflurane-induced ferroptotic neuronal death in SH-SY5Y cells via the 5' AMP-activated protein kinase/mammalian target of rapamycin pathway.

Annals of translational medicine

Cheng L,Zhu X,Liu Y,Zhu K,Lin K,Li F

44-1150G was used in Western Blot to show that downregulation of ACSL4 restrained sev-induced ferroptotic cell death via AMPK/mTOR signaling, providing the basis for an approach to alleviate sev-induced postoperative cognitive dysfunction (POCD).

Wed Sep 01 00:00:00 UTC 2021

3H-1,2-Dithiole-3-Thione Protects Lens Epithelial Cells against Fructose-Induced Epithelial-Mesenchymal Transition via Activation of AMPK to Eliminate AKR1B1-Induced Oxidative Stress in Diabetes Mellitus.

Antioxidants (Basel, Switzerland)

Wu TT,Chen YY,Ho CY,Yeh TC,Sun GC,Tseng CJ,Cheng PW

44-1150G was used in Western Blot to investigate how 3H-1,2-dithiole-3-thione (D3T) reduces epithelial-mesenchymal transition formation in lens epithelial cells of the fructose-induced diabetes mellitus (DM).

Tue Jul 06 00:00:00 UTC 2021

Antioxidants (Basel, Switzerland)

Wu TT,Chen YY,Ho CY,Yeh TC,Sun GC,Tseng CJ,Cheng PW

Tue Jul 06 00:00:00 UTC 2021

Evaluation of liver kinase B1 downstream signaling expression in various breast cancers and relapse free survival after systemic chemotherapy treatment.

Oncotarget

Nguyen K,Rivera A,Alzoubi M,Wathieu H,Dong S,Yousefi H,Matossian M,Alahari S,Drewry D,Burow M,Collins-Burow B

44-1150G was used in Western Blotting to evaluate LKB1-signaling pathway gene expression associations with patient survival in overall breast cancer.

Tue May 25 00:00:00 UTC 2021

AKR1B1-Induced Epithelial-Mesenchymal Transition Mediated by RAGE-Oxidative Stress in Diabetic Cataract Lens.

Antioxidants (Basel, Switzerland)

Wu TT,Chen YY,Chang HY,Kung YH,Tseng CJ,Cheng PW

44-1150G was used in Immunocytochemistry-Immunofluorescence to show that epithelial-mesenchymal transition in the DM cataract occurs through AKR1B1-enhanced AGE and RAGE-oxidative stress generation in lens epithelial cells from DM (+) cataract patients.

Wed Mar 25 00:00:00 UTC 2020

N-myristoyltransferase deficiency impairs activation of kinase AMPK and promotes synovial tissue inflammation.

Nature immunology

Wen Z,Jin K,Shen Y,Yang Z,Li Y,Wu B,Tian L,Shoor S,Roche NE,Goronzy JJ,Weyand CM

44-1150G was used in Flow Cytometry to find that RA T cells had a defect in NMT1 function, which prevented AMPK activation and enabled unopposed mTORC1 signaling.

Fri Mar 01 00:00:00 UTC 2019

Impaired Cellular Energy Metabolism Contributes to Duck-Enteritis-Virus-Induced Autophagy via the AMPK-TSC2-MTOR Signaling Pathway.

Frontiers in cellular and infection microbiology

Yin H,Zhao L,Li S,Xu L,Wang Y,Chen H

Published figure using Phospho-AMPK alpha-1,2 (Thr183, Thr172) polyclonal antibody (Product # 44-1150G) in Western Blot

Mon Apr 23 00:00:00 UTC 2018

生物信息学

蛋白别名: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha 2; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK-alpha-1 chain; AMPK-alpha-2 chain; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; pAMPK; prkaa2; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; Tau-protein kinase PRKAA1

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基因别名: 2310008I11Rik; A830082D05; AI194361; AI450832; AL024255; AMPK; AMPK1; AMPK2; AMPKa1; AMPKa2; AMPKalpha1; AMPKalpha2; C130083N04Rik; PRKAA; PRKAA1; PRKAA2