Invitrogen

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Recombinant Rabbit Monoclonal Antibody (10H2L20)

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody (701068) in ICC/IF

Immunofluorescence analysis of Phospho-AMPK alpha-1,2 (Thr183, Thr172) was performed using 90% confluent log phase A-431 cells treated with 1mmol/mL Metformin for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-AMPK alpha-1,2 (Thr183, Thr17... View More

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody

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产品信息

701068

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

10H2L20

抗原

Phosphopeptide corresponding to amino acids 168-178 of human AMPK alpha

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532369

产品详细信息

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha 2; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK-alpha-1 chain; AMPK-alpha-2 chain; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; pAMPK; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; Tau-protein kinase PRKAA1

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基因别名: AMPK; AMPK1; AMPK2; AMPKa1; AMPKa2; PRKAA; PRKAA1; PRKAA2

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UniProt ID: (Human) Q13131, (Human) P54646

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Entrez Gene ID: (Human) 5562, (Human) 5563

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